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Caspase-9 Colorimetric Assay Kit

Caspase-9 Colorimetric Assay Kit. GTX85538
Caspase-9 Colorimetric Assay Kit. GTX85538

Cat No. GTX85538

Package
100 assay ($389)
PRODUCT

Product Description

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-9 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate LEHD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm.

‧ Detection method- Spectrophotometer (OD-400- or 405-nm) or a microtiter plate reader
‧ Sample type- Cell and tissue lysates
‧ Species reactivity- Mammalian
‧ Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits
‧ Simple one-step procedure; takes 1-2 hours
‧ Fast and convenient
‧ Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-9 activity.

Kit Contents:
Cell Lysis Buffer
2X Reaction Buffer
LEHD-pNA (4 mM)
DTT (1 M)
Dilution Buffer

PROPERTIES

Storage

Store at -20ºC. Product has an expected shelf life of 6-12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 9 Activity Assay, Caspase substrate kit, Apoptosis Assay, Caspase9 Substrate, Caspase-9 Activity Assay, Caspase 9 Substrate, Caspase-9 Substrate, Caspase 9 Assay, Caspase-9 Assay, Caspase9 Assay, Caspase9 Activity Assay
DATA IMAGES
Caspase-9 Colorimetric Assay Kit. GTX85538

GTX85538 Image

Analysis of Caspase-9 Activity in Jurkat cells. Apoptosis was induced in Jurkat cells by camptothecin (2 μM) for 6 hours. Caspase activity was analyzed using Caspase-9 Colorimetric Assay Kit. Results were analyzed by spectrophotometer.

Caspase-9 Colorimetric Assay Kit. GTX85538

GTX85538 Image

10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.

REFERENCE
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Package List Price ($)
$ 389