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Caspase-9 Fluorometric Assay Kit

Caspase-9 Fluorometric Assay Kit. GTX85537
Caspase-9 Fluorometric Assay Kit. GTX85537

Cat No. GTX85537

100 assay ($459)


Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-9 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (max = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.

‧ Detection method- Flow cytometry(400-nm excitation filter and 505-nm emission filter), fluorecence microtiter plate reader and fluorescence microscopy
‧ Sample type- Cell and tissue lysates
‧ Species reactivity- Mammalian
‧ Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits
‧ Simple one-step procedure; takes 1-2 hours
‧ Fast and convenient
‧ Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-9 activity.

Kit Contents:
Cell Lysis Buffer
2X Reaction Buffer
DTT (1 M)



Store at -20ºC. Product has an expected shelf life of 6-12 months.


For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.



Caspase 9 Activity Assay , Caspase substrate kit , Apoptosis Assay , Caspase9 Substrate , Caspase-9 Activity Assay , Caspase 9 Substrate , Caspase-9 Substrate , Caspase 9 Assay , Caspase-9 Assay , Caspase9 Assay , Caspase9 Activity Assay


Caspase-9 Fluorometric Assay Kit. GTX85537

GTX85537 Image

Caspase-9 Fluorometric Assay Kit. GTX85537

GTX85537 Image

Example of GeneTex Fluorometric Caspase Activity Assay Kit: Analysis of Caspase-3 Activity in Jurkat cells. Apoptosis was induced in Jurkat cells by camptothecin (2 μM) for 6 hours. Caspase activity was analyzed using Caspase-9 Colorimetric Assay Kit. Results were analyzed by spectrophotometer.



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Package List Price ($)
$ 459