GeneTex
  • Country / Location Selection

United States (US)

Caspase-9 Fluorometric Assay Kit

Caspase-9 Fluorometric Assay Kit. GTX85537
Caspase-9 Fluorometric Assay Kit. GTX85537

Cat No. GTX85537

Package
100 assay ($379)
PRODUCT

Product Description

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-9 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (max = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.

‧ Detection method- Flow cytometry(400-nm excitation filter and 505-nm emission filter), fluorecence microtiter plate reader and fluorescence microscopy
‧ Sample type- Cell and tissue lysates
‧ Species reactivity- Mammalian
‧ Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits
‧ Simple one-step procedure; takes 1-2 hours
‧ Fast and convenient
‧ Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-9 activity.

Kit Contents:
Cell Lysis Buffer
2X Reaction Buffer
LEHD-AFC (1 mM)
DTT (1 M)

PROPERTIES

Storage

Store at -20ºC. Product has an expected shelf life of 6-12 months.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase 9 Activity Assay, Caspase substrate kit, Apoptosis Assay, Caspase9 Substrate, Caspase-9 Activity Assay, Caspase 9 Substrate, Caspase-9 Substrate, Caspase 9 Assay, Caspase-9 Assay, Caspase9 Assay, Caspase9 Activity Assay
DATA IMAGES
Caspase-9 Fluorometric Assay Kit. GTX85537

GTX85537 Image

10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.

Caspase-9 Fluorometric Assay Kit. GTX85537

GTX85537 Image

Example of GeneTex Fluorometric Caspase Activity Assay Kit: Analysis of Caspase-3 Activity in Jurkat cells. Apoptosis was induced in Jurkat cells by camptothecin (2 μM) for 6 hours. Caspase activity was analyzed using Caspase-9 Colorimetric Assay Kit. Results were analyzed by spectrophotometer.

REFERENCE
REVIEW
There are currently no reviews for Caspase-9 Fluorometric Assay Kit (GTX85537). Be the first to share your experience with this product.
Package List Price ($)
$ 379