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Catalase antibody [N2C1], Internal

Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149
Anti-Catalase antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113149
Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149
Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149

Cat No. GTX113149

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

Molt-4 , Raji , rat kidney , CAT-transfected 293T

Predict Reactivity

Mouse, Zebrafish, Bovine, Dog, Chicken, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Catalase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949850

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

catalase

Cellular Localization

Peroxisome

Background

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149

GTX113149 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Catalase antibody (GTX113149) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Catalase antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113149

GTX113149 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Catalase(GTX113149) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149

GTX113149 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
B: Raji
7.5% SDS PAGE
GTX113149 diluted at 1:1000

Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149

GTX113149 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Catalase antibody (GTX113149) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for Catalase antibody [N2C1], Internal (GTX113149). Be the first to share your publications with this product.

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169