APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Molt-4 , Raji , CAT-transfected 293T , rat kidney
Predict Reactivity
Mouse, Bovine, Dog, Chicken, Guinea pig, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Catalase. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949850
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
catalase
Cellular Localization
Peroxisome
Background
This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Catalase antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113149](/templates/default/js/lightbox/images/loading.gif "Anti-Catalase antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113149")
|
GTX113149 IHC-P Image
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Catalasealase antibody [N2C1], Internal (GTX113149) at 1:300 dilution.
|
|
|
GTX113149 WB Image
Sample (30 μg of whole cell lysate)
A: zebrafish muscle
7.5% SDS PAGE
GTX113149 diluted at 1:1000
|
|
|
GTX113149 WB Image
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Catalase antibody (GTX113149) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX113149 IHC-P Image
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Catalase(GTX113149) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
|
GTX113149 WB Image
Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
B: Raji
7.5% SDS PAGE
GTX113149 diluted at 1:1000
|
|
|
GTX113149 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Catalase antibody (GTX113149) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for Catalase antibody [N2C1], Internal (GTX113149). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Catalase antibody [N2C1], Internal (GTX113149). Be the first to share your experience with this product.
![Anti-Catalase antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113149](/upload/website/prouct_img/small/GTX113149/GTX113149_40086_IHC_Z_22111423_487.webp "Anti-Catalase antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113149")
![Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149](/upload/website/prouct_img/small/GTX113149/GTX113149_40086_WB_Z_22111423_264.webp "Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149")
![Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149](/upload/website/prouct_img/small/GTX113149/GTX113149_40086_20170504_WB_R_kidney_w_23060500_223.webp "Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149")
![Anti-Catalase antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113149](/upload/website/prouct_img/small/GTX113149/GTX113149_40086_IHC_w_23060501_239.webp "Anti-Catalase antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113149")
![Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149](/upload/website/prouct_img/small/GTX113149/GTX113149_WB_w_23060501_131.webp "Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149")
![Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149](/upload/website/prouct_img/small/GTX113149/GTX113149_40086_20170504_WB_B_w_23060501_776.webp "Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149")
![Anti-Catalase antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113149](/upload/website/prouct_img/normal/GTX113149/GTX113149_40086_IHC_Z_22111423_487.webp "Anti-Catalase antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113149")
![Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149](/upload/website/prouct_img/normal/GTX113149/GTX113149_40086_WB_Z_22111423_264.webp "Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149")
![Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149](/upload/website/prouct_img/normal/GTX113149/GTX113149_40086_20170504_WB_R_kidney_w_23060500_223.webp "Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149")
![Anti-Catalase antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113149](/upload/website/prouct_img/normal/GTX113149/GTX113149_40086_IHC_w_23060501_239.webp "Anti-Catalase antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113149")
![Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149](/upload/website/prouct_img/normal/GTX113149/GTX113149_WB_w_23060501_131.webp "Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149")
![Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149](/upload/website/prouct_img/normal/GTX113149/GTX113149_40086_20170504_WB_B_w_23060501_776.webp "Anti-Catalase antibody [N2C1], Internal used in Western Blot (WB). GTX113149")