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Catalase antibody

Orthogonal Overexpression
Anti-Catalase antibody used in Western Blot (WB). GTX110704
Anti-Catalase antibody used in Western Blot (WB). GTX110704
Anti-Catalase antibody used in Western Blot (WB). GTX110704
Anti-Catalase antibody used in IHC (Paraffin sections) (IHC-P). GTX110704
Anti-Catalase antibody used in Western Blot (WB). GTX110704
Anti-Catalase antibody used in Western Blot (WB). GTX110704
Anti-Catalase antibody used in Western Blot (WB). GTX110704
Anti-Catalase antibody used in Western Blot (WB). GTX110704
Anti-Catalase antibody used in Western Blot (WB). GTX110704
Anti-Catalase antibody used in Western Blot (WB). GTX110704
Anti-Catalase antibody used in Western Blot (WB). GTX110704
Anti-Catalase antibody used in Western Blot (WB). GTX110704
Anti-Catalase antibody used in Western Blot (WB). GTX110704
Anti-Catalase antibody used in Western Blot (WB). GTX110704
Anti-Catalase antibody used in Western Blot (WB). GTX110704
Anti-Catalase antibody used in Western Blot (WB). GTX110704
Anti-Catalase antibody used in Western Blot (WB). GTX110704

Cat. No. GTX110704

Reference 62

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Catalase products
PRODUCT

Summary

Catalase antibody detects catalase protein, an antioxidant enzyme (predicted molecular weight of 60 kDa). Mutation of the CAT gene that codes for catalase protein can result in acatalasemia. This catalase antibody (GTX110704) is a well-known reagent cited in multiple publications.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

CAT-transfected 293T

Predict Reactivity

Zebrafish, Drosophila, Bovine, Dog, Chicken, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Catalase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949848

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

catalase

Cellular Localization

Peroxisome

Background

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Catalase antibody (GTX110704) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Catalase antibody (GTX110704) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Catalase antibody (GTX110704) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Catalase antibody used in IHC (Paraffin sections) (IHC-P). GTX110704

GTX110704 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Catalase (GTX110704) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

Orthogonal

The data was published in the journal Reprod Biol Endocrinol in 2018.PMID: 29316934

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

Orthogonal

The data was published in the journal BMC Res Notes in 2015.PMID: 26423686

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

Orthogonal

The data was published in the journal Reprod Biol Endocrinol in 2018.PMID: 29316934

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

Orthogonal

The data was published in the journal Nutrients in 2018.PMID: 30200495

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

The data was published in the journal PLoS One in 2016. PMID: 27537547

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

The data was published in the journal Physiol Rep in 2016.PMID: 27884954

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

The data was published in the journal Reprod Biol Endocrinol in 2018.PMID: 29316934

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

The data was published in the journal Physiol Rep in 2015. PMID: 26603455

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

The data was published in the journal Front Physiol in 2016. PMID: 27877138

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

The data was published in the journal Front Physiol in 2019. PMID: 31040795

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

The data was published in the journal Heliyon in 2018. PMID: 30533548

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

The data was published in the journal Heliyon in 2018. PMID: 30533548

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

The data was published in the journal Heliyon in 2018. PMID: 30533548

REFERENCE

Application Reference

J Mallard et al. J Cachexia Sarcopenia Muscle 2024; 15 (1) : 292-305 A single chemotherapy administration induces muscle atrophy, mitochondrial alterations and apoptosis in breast cancer patients
Application : WB
H?rle L et al. Sci Rep 2023; 13 (1) : 20390 Hepatocyte integrity depends on c-Jun-controlled proliferation in Schistosoma?mansoni infected mice.
Kogami M et al. Oncol Lett 2023; 26 (1) : 313
Mariko Kogami et al. Oncol. Lett. 2023; 26 : 313
Application : WB
Reactivity : Human
Ramos H et al. Antioxidants (Basel) 2022; 11 (7) : 0 Antioxidant Effects of DPP-4 Inhibitors in Early Stages of Experimental Diabetic Retinopathy.
Application : WB
Reactivity : Mouse
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REVIEW

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Application Reference
J Mallard et al. J Cachexia Sarcopenia Muscle 2024; 15 (1):292-305 A single chemotherapy administration induces muscle atrophy, mitochondrial alterations and apoptosis in breast cancer patients
Application : WB
H?rle L et al. Sci Rep 2023; 13 (1):20390 Hepatocyte integrity depends on c-Jun-controlled proliferation in Schistosoma?mansoni infected mice.
Kogami M et al. Oncol Lett 2023; 26 (1):313
Mariko Kogami et al. Oncol. Lett. 2023; 26:313
Application : WB
Reactivity : Human
Ramos H et al. Antioxidants (Basel) 2022; 11 (7):0 Antioxidant Effects of DPP-4 Inhibitors in Early Stages of Experimental Diabetic Retinopathy.
Application : WB
Reactivity : Mouse
Kornelius E et al. Biomolecules 2022; 12 (10) Liraglutide Attenuates Glucolipotoxicity-Induced RSC96 Schwann Cells' Inflammation and Dysfunction.
Osuru HP et al. Front Cardiovasc Med 2022; 9:711421 Molecular and Cellular Response of the Myocardium (H9C2 Cells) Towards Hypoxia and HIF-1α Inhibition.
Kelley RC et al. J Appl Physiol (1985) 2022; 132 (1):106-125 Skeletal myopathy in a rat model of postmenopausal heart failure with preserved ejection fraction.
Application : WB
Reactivity : Rat
Mesquita PHC et al. Physiol Rep 2022; 10 (24):e15542 Effects of aging and long-term physical activity on mitochondrial physiology and redox state of the cortex and cerebellum of female rats.
Joris Mallard et al. J Cachexia Sarcopenia Muscle 2022; 13 (3):1896-1907 Chemotherapy impairs skeletal muscle mitochondrial homeostasis in early breast cancer patients.
Application : WB
Reactivity : Human
Zhao X et al. J Microbiol Biotechnol 2021; 31 (8):1163-1174 Antioxidant Activity of Novel Casein-Derived Peptides with Microbial Proteases as Characterized via Keap1-Nrf2 Pathway in HepG2 Cells.
Sigrist-Flores SC et al. Toxicol Res (Camb) 2021; 10 (4):817-823 Variation in resistance to oxidative stress in Oregon-(R)R-flare and Canton-S strains of Drosophila melanogaster.
Hwang JS et al. Biomed Pharmacother 2021; 143:112223 Peroxisome proliferator-activated receptor δ rescues xCT-deficient cells from ferroptosis by targeting peroxisomes.
Application : WB
Reactivity : Mouse
Chien LH et al. Antioxidants (Basel) 2021; 10 (10)
Application : WB
Reactivity : Mouse
Hwang JS et al. Antioxidants (Basel) 2021; 10 (8) Catalase Mediates the Inhibitory Actions of PPARδ against Angiotensin II-Triggered Hypertrophy in H9c2 Cardiomyocytes.
Application : WB
Reactivity : Rat
Waditee-Sirisattha R et al. J Photochem Photobiol B 2021; 223:112296 Protective effects of mycosporine-like amino acid-containing emulsions on UV-treated mouse ear tissue from the viewpoints of antioxidation and antiglycation.
Application : WB
Reactivity : Mouse
Jiang WP et al. Antioxidants (Basel) 2021; 10 (6) Sanghuangporus sanghuang Mycelium Prevents Paracetamol-Induced Hepatotoxicity through Regulating the MAPK/NF-κB, Keap1/Nrf2/HO-1, TLR4/PI3K/Akt, and CaMKKβ/LKB1/AMPK Pathways and Suppressing Oxidative Stress and Inflammation.
Application : WB
Reactivity : Mouse
Chang HM et al. Antioxidants (Basel) 2021; 10 (5) Melatonin Successfully Rescues the Hippocampal Molecular Machinery and Enhances Anti-oxidative Activity Following Early-Life Sleep Deprivation Injury.
Application : WB
Reactivity : Rat
Mesquita PHC et al. Antioxidants (Basel) 2021; 10 (3) Effects of Resistance Training on the Redox Status of Skeletal Muscle in Older Adults.
Application : WB
Reactivity : Human
Dai J et al. Elife 2021; 10 Curcumin promotes AApoAII amyloidosis and peroxisome proliferation in mice by activating the PPARα signaling pathway.
Application : WB, IHC-P
Reactivity : Mouse
Murata K et al. J Nat Med 2021; 75 (1):142-155 Oral administration of Jumihaidokuto inhibits UVB-induced skin damage and prostaglandin E2 production in HR-1 hairless mice.
Application : IHC-Fr
Reactivity : Mouse
Adam Piecuch et al. Prz Gastroenterol 2020; 15 (4):330-337 Catalase immunoexpression in colorectal lesions.
Application : IHC-P
Reactivity : Human
Park NR et al. J Comp Physiol B 2020; 190 (4):465-477 Mitochondrial physiology varies with parity and body mass in the laboratory mouse (Mus musculus).
Application : WB
Reactivity : Mouse
Huang CY et al. Nutrients 2020; 12 (6) Attenuation of Lipopolysaccharide-Induced Acute Lung Injury by Hispolon in Mice, Through Regulating the TLR4/PI3K/Akt/mTOR and Keap1/Nrf2/HO-1 Pathways, and Suppressing Oxidative Stress-Mediated ER Stress-Induced Apoptosis and Autophagy.
Application : WB
Reactivity : Mouse
Du G et al. Dev Cell 2020; 53 (2):169-184.e11 Peroxisome Elevation Induces Stem Cell Differentiation and Intestinal Epithelial Repair.
Application : WB
Reactivity : Human
Arc-Chagnaud C et al. Front Physiol 2020; 11:71 Evaluation of an Antioxidant and Anti-inflammatory Cocktail Against Human Hypoactivity-Induced Skeletal Muscle Deconditioning.
Application : IHC-P
Reactivity : Mouse
Zhou H et al. JCI Insight 2019; 5 Targeting ATGL to rescue BSCL2 lipodystrophy and its associated cardiomyopathy.
Parry HA et al. Front Physiol 2019; 10:436 Bovine Milk Extracellular Vesicles (EVs) Modification Elicits Skeletal Muscle Growth in Rats.
Huang SY et al. Biomolecules 2019; 9 (8) The Protective Effect of Hispidin against Hydrogen Peroxide-Induced Oxidative Stress in ARPE-19 Cells via Nrf2 Signaling Pathway.
Application : WB
Reactivity : Human
Wu CT et al. Oxid Med Cell Longev 2019; 2019:9056845 Salvianolic Acid C against Acetaminophen-Induced Acute Liver Injury by Attenuating Inflammation, Oxidative Stress, and Apoptosis through Inhibition of the Keap1/Nrf2/HO-1 Signaling.
Application : WB
Reactivity : Mouse
Morton AB et al. Redox Biol 2019; 20:402-413 Increased SOD2 in the diaphragm contributes to exercise-induced protection against ventilator-induced diaphragm dysfunction.
Application : WB
Reactivity : Rat
Jihong Wu et al. RSC Adv 2018; 8 (20):10898-10906 Cytoprotective effects of a tripeptide from Chinese Baijiu against AAPH-induced oxidative stress in HepG2 cells via Nrf2 signaling.
Application : WB
Reactivity : Human
Hyatt HW et al. Reprod Biol Endocrinol 2018; 16 (1):2 Physiological, mitochondrial, and oxidative stress differences in the presence or absence of lactation in rats.
Hsieh YH et al. Nutrients 2018; 10 (9) Ginsenoside Rh2 Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Regulating the TLR4/PI3K/Akt/mTOR, Raf-1/MEK/ERK, and Keap1/Nrf2/HO-1 Signaling Pathways in Mice.
Parry HA et al. Heliyon 2018; 4 (11):e00975 Ketogenic diet increases mitochondria volume in the liver and skeletal muscle without altering oxidative stress markers in rats.
Dongrui Zhao et al. RSV Adv. 2018; 8:35474-35484 Quantification and cytoprotection by vanillin, 4-methylguaiacol and 4-ethylguaiacol against AAPH-induced abnormal oxidative stress in HepG2 cells.
Application : WB
Reactivity : Human
Pei-Ying Li et al. RSC Adv. 2018; 8:31515-31528 Alpinumisoflavone attenuates lipopolysaccharide-induced acute lung injury by regulating the effects of anti-oxidation and anti-inflammation both in vitro and.
Application : WB
Reactivity : Mouse
Lee CT et al. J Steroid Biochem Mol Biol 2018; (Epub) 1,25-dihydroxyvitamin d3 modulates the effects of sublethal bpa on mitochondrial function via activating pi3k-akt pathway and 17β-* secretion in rat granulosa cells.
Application : WB
Reactivity : Rat
Wu CC et al. Redox Biol 2018; 19:74-80 Upregulation of Znf179 acetylation by SAHA protects cells against oxidative stress.
Application : WB
Reactivity : Mouse
Hyatt HW et al. Reprod Sci 2018;:1.93E+15 Changes in Metabolism, Mitochondrial Function, and Oxidative Stress Between Female Rats Under Nonreproductive and 3 Reproductive Conditions.
Application : WB
Reactivity : Rat
Zhang Y et al. J Exp Biol 2018; (Epub) High activity before breeding improves reproductive performance by enhancing mitochondrial function and biogenesis.
Application : WB
Reactivity : Mouse
Wu YL et al. Free Radic Biol Med 2018; 115:309-317 Caffeic acid and resveratrol ameliorate cellular damage in cell and Drosophila models of spinocerebellar ataxia type 3 through upregulation of Nrf2 pathway.
Application : WB
Reactivity : Human
Noguera NI et al. Oncotarget 2017; 8 (20):32550-32565 high-dose ascorbate and * selectively kill acute myeloid leukemia and acute promyelocytic leukemia blasts in vitro.
Hyatt HW et al. Sci Rep 2017; 7 (1):17118 Lactation has persistent effects on a mother's metabolism and mitochondrial function.
Application : WB
Reactivity : Rat
Haun CT et al. Eur J Appl Physiol 2017; 117 (12):2587-2600 Concomitant external pneumatic compression treatment with consecutive days of high intensity interval training reduces markers of proteolysis.
Application : WB
Reactivity : Human
Zhang Y et al. Am J Physiol Regul Integr Comp Physiol 2017;:ajpregu.00176.2017 A mitohormetic response to pro-oxidant exposure in the house mouse.
Application : WB
Reactivity : Mouse
Kephart WC et al. Nutrients 2017; 9 (9) The 1-Week and 8-Month Effects of a Ketogenic Diet or Ketone Salt Supplementation on Multi-Organ Markers of Oxidative Stress and Mitochondrial Function in Rats.
Application : WB
Reactivity : Rat
Chao W et al. Int Immunopharmacol 2017; 50:77-86 3, 4-dihydroxybenzalacetone attenuates lipopolysaccharide-induced inflammation in acute lung injury via down-regulation of MMP-2 and MMP-9 activities through suppressing ROS-mediated MAPK and PI3K/AKT signaling pathways.
Application : WB
Reactivity : Mouse
Lin WC et al. RSC Adv. 2017; 7:21234-21251 Anti-inflammatory activity of Sanghuangporus sanghuang by suppressing the TLR4-mediated PI3K/AKT/mTOR/IKKβ signaling pathway.
Application : WB
Reactivity : Mouse
Mowry A.V. et al. Ecology and Evolution 2017; (Epub) Mitochondrial function and bioenergetic trade-offs during lactation in the house mouse (Mus musculus).
Application : WB
Reactivity : Mouse
Chao W et al. Molecules 2017; 22 (4) 3,4-Dihydroxybenzalactone Suppresses Human Non-Small Cell Lung Carcinoma Cells Metastasis via Suppression of Epithelial to Mesenchymal Transition, ROS-Mediated PI3K/AKT/MAPK/MMP and NFκB Signaling Pathways.
Application : WB
Reactivity : Human
Martin JS et al. Physiol Rep 2016; 4 (22) Impact of external pneumatic compression target inflation pressure on transcriptome-wide RNA expression in skeletal muscle.
Hyatt HW et al. Front Physiol 2016; 7:533 A Ketogenic Diet in Rodents Elicits Improved Mitochondrial Adaptations in Response to Resistance Exercise Training Compared to an Isocaloric Western Diet.
Hyatt HW et al. Int J Physiol Pathophysiol Pharmacol 2016; 8 (4):160-168 Comparative changes in antioxidant enzymes and oxidative stress in cardiac, fast twitch and slow twitch skeletal muscles following endurance exercise training.
Xu YM et al. Oncotarget 2016; 7 (5):6146-58 Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
Reactivity : Human
Hyatt HW et al. Int J Physiol Pathophysiol Pharmacol 2016; 8 (4):160-168 Comparative changes in antioxidant enzymes and oxidative stress in cardiac, fast twitch and slow twitch skeletal muscles following endurance exercise training.
Application : WB
Reactivity : Rat
Hyatt H.W. et al. Front. Physiol. 2016; (Epub) A Ketogenic Diet in Rodents Elicits Improved Mitochondrial Adaptations in Response to Resistance Exercise Training Compared to an Isocaloric Western Diet.
Application : WB
Reactivity : Rat
Dai J et al. J Biol Inorg Chem 2016; 21 (8):1037-1046 Selenite and ebselen supplementation attenuates D-galactose-induced oxidative stress and increases expression of SELR and SEP15 in rat lens.
Application : WB
Reactivity : Rat
Mowry AV et al. PLoS One 2016; 11 (8):e0160883 Reproduction Does Not Adversely Affect Liver Mitochondrial Respiratory Function but Results in Lipid Peroxidation and Increased Antioxidants in House Mice.
Application : WB
Reactivity : Mouse
Hyatt HW et al. Physiol Rep 2015; 3 (11) Comparative adaptations in oxidative and glycolytic muscle fibers in a low voluntary wheel running rat model performing three levels of physical activity.
Holland AM et al. BMC Res Notes 2015; 8:514 Influence of endurance exercise training on antioxidant enzymes, tight junction proteins, and inflammatory markers in the rat ileum.
Wu CH et al. J Biol Chem 2012; 287 (30):25292-302 Nickel-induced epithelial-mesenchymal transition by reactive oxygen species generation and E-cadherin promoter hypermethylation.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169