GeneTex
  • Country / Location Selection

United States (US)

Catalase antibody

Anti-Catalase antibody used in IHC (Paraffin sections) (IHC-P). GTX110704
Anti-Catalase antibody used in Western Blot (WB). GTX110704
Anti-Catalase antibody used in Western Blot (WB). GTX110704
Anti-Catalase antibody used in Western Blot (WB). GTX110704

Cat No. GTX110704

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)
PRODUCT

Summary

Catalase antibody detects catalase protein, an antioxidant enzyme (predicted molecular weight of 60 kDa). Mutation of the CAT gene that codes for catalase protein can result in acatalasemia. This catalase antibody (GTX110704) is a well-known reagent cited in multiple publications.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

CAT-transfected 293T , mouse kidney , rat kidney

Predict Reactivity

Zebrafish, Drosophila, Bovine, Dog, Chicken, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Catalase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949848

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

catalase

Cellular Localization

Peroxisome

Background

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Catalase antibody used in IHC (Paraffin sections) (IHC-P). GTX110704

GTX110704 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Catalase (GTX110704) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Catalase antibody (GTX110704) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Catalase antibody (GTX110704) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Catalase antibody used in Western Blot (WB). GTX110704

GTX110704 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Catalase antibody (GTX110704) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Catalase antibody Cat No. GTX110704

Rating ( Average 5 based on 13 users reviews)
Application
Western Blot(WB) ( Average 5 based on 13 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Stem Cell Res Ther in 2017. PMID: 28482861
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Mol Cancer in 2017. PMID: 28784136
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Front Neurol in 2017. PMID: 28713327
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Front Pharmacol in 2017. PMID: 29184499
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 31757960
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 31757960
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Physiol Rep in 2015. PMID: 26603455
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Front Physiol in 2016. PMID: 27877138
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016. PMID: 27537547
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Front Physiol in 2019. PMID: 31040795
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Heliyon in 2018. PMID: 30533548
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Heliyon in 2018. PMID: 30533548
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Heliyon in 2018. PMID: 30533548
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169