GeneTex
United States (US)

Cav beta 4 antibody [C3], C-term

GTX100202_39391_WB_M_cerebellum_19011506_593.jpg
GTX100202_39391_IFA_19011506_124.jpg
GTX100202_39391_IHC_19011506_976.jpg
GTX100202_39391_WB_19011506_636.jpg
GTX100202_39391_20170608_IHC-P_M_19011506_900.jpg

Cat No. GTX100202

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , HepG2 , A375 , mouse cerebellum

Predict Reactivity

Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CACNB4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Calcium Voltage-Gated Channel Auxiliary Subunit Beta 4,Cab4,Cacnlb4,Ea5,Eig9,Ejm,Ejm4,Ejm6,Cacnb4

Background

This gene encodes a member of the beta subunit family of voltage-dependent calcium channel complex proteins. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. The protein encoded by this locus plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Certain mutations in this gene have been associated with idiopathic generalized epilepsy (IGE) and juvenile myoclonic epilepsy (JME). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100202_39391_WB_M_cerebellum_19011506_593.jpg

GTX100202 WB Image

Sample (50 ug of whole cell lysate)
A: mouse cerebellum
7.5% SDS PAGE
GTX100202 diluted at 1:1000

GTX100202_39391_IFA_19011506_124.jpg

GTX100202 ICC/IF Image

CACNB4 antibody [C3], C-term detects CACNB4 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CACNB4 protein stained by CACNB4 antibody [C3], C-term (GTX100202) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100202_39391_IHC_19011506_976.jpg

GTX100202 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using CACNB4(GTX100202) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100202_39391_WB_19011506_636.jpg

GTX100202 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: HepG2
C: A375
7.5% SDS PAGE
GTX100202 diluted at 1:5000

GTX100202_39391_20170608_IHC-P_M_19011506_900.jpg

GTX100202 IHC-Fr Image

Cav beta 4 antibody [C3], C-term detects Cav beta 4 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Cav beta 4 protein stained by Cav beta 4 antibody [C3], C-term (GTX100202) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169