APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , HepG2 , A375 , mouse cerebellum
Predict Reactivity
Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CACNB4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
CAB4 antibody, CACNLB4 antibody, EA5 antibody, EIG9 antibody, EJM antibody, EJM4 antibody, EJM6 antibody, CACNB4 antibody, "dihydropyridine-sensitive L-type, calcium channel beta-4 subunit antibody", calcium channel voltage-dependent subunit beta 4 antibody, voltage-dependent L-type calcium channel subunit beta-4 antibody, "calcium channel, voltage-dependent, beta 4 subunit antibody"
Background
This gene encodes a member of the beta subunit family of voltage-dependent calcium channel complex proteins. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. The protein encoded by this locus plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Certain mutations in this gene have been associated with idiopathic generalized epilepsy (IGE) and juvenile myoclonic epilepsy (JME). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX100202 WB Image
Sample (50 ug of whole cell lysate)
A: mouse cerebellum
7.5% SDS PAGE
GTX100202 diluted at 1:1000
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GTX100202 ICC/IF Image
CACNB4 antibody [C3], C-term detects CACNB4 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CACNB4 protein stained by CACNB4 antibody [C3], C-term (GTX100202) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX100202 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human hepatoma, using CACNB4(GTX100202) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100202 WB Image
Sample (30 ug of whole cell lysate)
A: 293T
B: HepG2
C: A375
7.5% SDS PAGE
GTX100202 diluted at 1:5000
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GTX100202 IHC-Fr Image
Cav beta 4 antibody [C3], C-term detects Cav beta 4 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Cav beta 4 protein stained by Cav beta 4 antibody [C3], C-term (GTX100202) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
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