GeneTex
United States (US)

Caveolin 2 antibody

GTX108294_IHC_19011507_856.jpg
GTX108294_WB_19011507_262.jpg
GTX108294_39778_IFA_19011507_946.jpg
GTX108294_39778_20151029_WB_R_muscle_19011507_673.jpg
GTX108294_39778_20151029_WB_M_19011507_603.jpg
GTX108294_39778_IP_19011507_793.jpg

Cat No. GTX108294

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

A549 , C8D30 , Rat muscle

Predict Reactivity

Rabbit, Sheep, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Caveolin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caveolin 2,Cav,Cav2

Cellular Localization

Golgi apparatus membrane; Single-pass membrane protein , Cell membrane; Single-pass membrane protein , Membrane , caveola; Single-pass membrane protein , Golgi apparatus membrane , Cell membrane , Membrane , caveola

Background

The protein encoded by this gene is a major component of the inner surface of caveolae, small invaginations of the plasma membrane, and is involved in essential cellular functions, including signal transduction, lipid metabolism, cellular growth control and apoptosis. This protein may function as a tumor suppressor. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Two transcript variants encoding distinct isoforms have been identified for this gene. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by one transcript. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108294_IHC_19011507_856.jpg

GTX108294 IHC-P Image

Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using Caveolin 2(GTX108294) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108294_WB_19011507_262.jpg

GTX108294 WB Image

Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
GTX108294 diluted at 1:1000

GTX108294_39778_IFA_19011507_946.jpg

GTX108294 ICC/IF Image

Caveolin 2 antibody detects Caveolin 2 protein at membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Caveolin 2 protein stained by Caveolin 2 antibody (GTX108294) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX108294_39778_20151029_WB_R_muscle_19011507_673.jpg

GTX108294 WB Image

Caveolin 2 antibody detects Caveolin 2 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody (GTX108294) diluted at 1:1000.

GTX108294_39778_20151029_WB_M_19011507_603.jpg

GTX108294 WB Image

Caveolin 2 antibody detects Caveolin 2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody (GTX108294) diluted at 1:1000.

GTX108294_39778_IP_19011507_793.jpg

GTX108294 IP Image

Caveolin 2 antibody immunoprecipitates caveolin 2 protein in IP experiments.
IP samples: A549 whole cell extract
A. 30 μg A549 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of caveolin 2 protein by 4 μg Caveolin 2 antibody (GTX108294)
15 % SDS-PAGE
The immunoprecipitated caveolin 2 protein was detected by Caveolin 2 antibody (GTX108294) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169