APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , C8D30 , rat muscle
Predict Reactivity
Rabbit, Sheep, Bovine, Cat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.94 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Caveolin 2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949852
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
caveolin 2 , CAV
Cellular Localization
Golgi apparatus membrane; Single-pass membrane protein , Cell membrane; Single-pass membrane protein , Membrane , caveola; Single-pass membrane protein , Golgi apparatus membrane , Cell membrane , Membrane , caveola
Background
The protein encoded by this gene is a major component of the inner surface of caveolae, small invaginations of the plasma membrane, and is involved in essential cellular functions, including signal transduction, lipid metabolism, cellular growth control and apoptosis. This protein may function as a tumor suppressor. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Two transcript variants encoding distinct isoforms have been identified for this gene. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by one transcript. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX108294 WB Image
Caveolin 2 antibody detects Caveolin 2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody (GTX108294) diluted at 1:1000.
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GTX108294 IP Image
Caveolin 2 antibody immunoprecipitates caveolin 2 protein in IP experiments.
IP samples: A549 whole cell extract
A. 30 μg A549 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of caveolin 2 protein by 4 μg Caveolin 2 antibody (GTX108294)
15 % SDS-PAGE
The immunoprecipitated caveolin 2 protein was detected by Caveolin 2 antibody (GTX108294) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX108294 WB Image
Caveolin 2 antibody detects Caveolin 2 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody (GTX108294) diluted at 1:1000.
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GTX108294 WB Image
Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
GTX108294 diluted at 1:1000
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GTX108294 IHC-P Image
Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using Caveolin 2(GTX108294) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX108294 ICC/IF Image
Caveolin 2 antibody detects Caveolin 2 protein at membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Caveolin 2 protein stained by Caveolin 2 antibody (GTX108294) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX108294 WB Image
The data was published in the journal Sci Rep in 2016.PMID: 27122250
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REFERENCE
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