30 mg of lysates from LNCaP cells expressing caveolin-2 were incubated without (lane 1) or with calf intestinal phosphatase (CIP; lane 2) resolved on 12% SDS-PAGE, transferred onto nitrocellulose membrane, and western blotted with the following caveolin-2 specific antibodies: total caveolin-2 (non-phospho serine 23 GTX13542; top panel), phospho-serine 23 (GTX13543; middle panel), and phospho-serine 36 (GTX13544;  bottom panel)