APPLICATION
Application Note
WB: Use at a concentration of 0.1 - 1 μg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 18.2 kDa). Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
Product Note
This antibody is specific for Caveolin 2 phosphorylated at serine 36 and does not detect non-phosphorylated Caveolin 2.
PROPERTIES
Form
Liquid
Buffer
50% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human synthetic peptide corresponding to amino acids 32-45 of caveolin 2 phosphorylated on serine 36.
Purification
Immunogen affinity purified
The affinity purified product has been cross adsorbed against the non phosphorylated peptide to remove antibodies that may detect the non phosphorylated form of the protein.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
caveolin 2 , CAV
Cellular Localization
Nucleus , Cytoplasm
Background
The protein encoded by this gene is a major component of the inner surface of caveolae, small invaginations of the plasma membrane, and is involved in essential cellular functions, including signal transduction, lipid metabolism, cellular growth control and apoptosis. This protein may function as a tumor suppressor. This gene and related family member (CAV1) are located next to each other on chromosome 7, and express colocalizing proteins that form a stable hetero-oligomeric complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Additional isoforms resulting from the use of alternate in-frame translation initiation codons have also been described, and shown to have preferential localization in the cell (PMID:11238462). [provided by RefSeq, May 2011]
Database
Research Area
DATA IMAGES
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GTX13544 WB Image
30 mg of lysates from LNCaP cells expressing caveolin-2 were incubated without (lane 1) or with calf intestinal phosphatase (CIP; lane 2) resolved on 12% SDS-PAGE, transferred onto nitrocellulose membrane, and western blotted with the following caveolin-2 specific antibodies: total caveolin-2 (non-phospho serine 23 GTX13542; top panel), phospho-serine 23 (GTX13543; middle panel), and phospho-serine 36 (GTX13544; bottom panel)
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REFERENCE
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REVIEW
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