Immunohistochemical analysis of paraffin-embedded SCM-1 xenograft, using Chk1(GTX100070) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

CHK1 antibody [C1C2-6], Internal detects Chk1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Chk1 protein stained by CHK1 antibody [C1C2-6], Internal (GTX100070) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.