Wild-type (WT) and Chk2 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Chk2 antibody [4B8] (GTX70100) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

CHEK2 antibody detects CHEK2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CHEK2 antibody (GTX70100) diluted at 1:500.

Immunoprecipitation of Chk2 protein from 293T whole cell extracts using 5 μg of Chk2 antibody (GTX70100).
Western blot analysis was performed using Chk2 antibody (GTX70100).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.