Choline Acetyltransferase antibody [N1N3]

Name: Choline Acetyltransferase antibody [N1N3]
Brand: GeneTex
SKU: GTX113164
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Choline Acetyltransferase antibody [N1N3] used in IHC (Frozen sections) (IHC-Fr). GTX113164

Anti-Choline Acetyltransferase antibody [N1N3] used in Western Blot (WB). GTX113164

Anti-Choline Acetyltransferase antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113164

Anti-Choline Acetyltransferase antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113164

Cat. No. GTX113164

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Choline Acetyltransferase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000

Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Choline Acetyltransferase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949973

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

choline O-acetyltransferase , CHOACTASE , CMS1A , CMS1A2 , CMS6

Background

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq]

Database

Gene ID: 1103 CHAT
UniProt: P28329 CHAT

Research Area

DATA IMAGES

GTX113164 IHC-Fr Image

Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein in the amacrine cells by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Choline Acetyltransferase protein stained by Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113164 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113164 IHC-Fr Image

Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Red: Choline Acetyltransferase stained by Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:300.
Blue: Hoechst 33342 staining.

GTX113164 ICC/IF Image

Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Choline Acetyltransferase protein stained by Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX113164 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113164 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113164 IHC-P Image

Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: Choline Acetyltransferase stained by Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:615.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113164 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ben-Zvi H et al. Int J Mol Sci 2022; 23 (24) PLEKHM2 Loss of Function Impairs the Activity of iPSC-Derived Neurons via Regulation of Autophagic Flux.

Kinga Moln?r et al. J Neuroinflammation 2022; 19 (1) : 68 Motoneuronal inflammasome activation triggers excessive neuroinflammation and impedes regeneration after sciatic nerve injury.

Application : IHC-Fr

Reactivity : Mouse

Bern?t N?gr?di et al. Front Pharmacol 2020; 11 : 584184 Upregulation of Nucleotide-Binding Oligomerization Domain-, LRR- and Pyrin Domain-Containing Protein 3 in Motoneurons Following Peripheral Nerve Injury in Mice.

Application : IHC-Fr

Reactivity : Mouse

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