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Choline Acetyltransferase antibody [N1N3]

Anti-Choline Acetyltransferase antibody [N1N3] used in IHC (Frozen sections) (IHC-Fr). GTX113164
Anti-Choline Acetyltransferase antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113164
Anti-Choline Acetyltransferase antibody [N1N3] used in Western Blot (WB). GTX113164
Anti-Choline Acetyltransferase antibody [N1N3] used in Western Blot (WB). GTX113164
Anti-Choline Acetyltransferase antibody [N1N3] used in Western Blot (WB). GTX113164
Anti-Choline Acetyltransferase antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113164
Anti-Choline Acetyltransferase antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113164

Cat No. GTX113164

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

IMR32 , mouse brain , rat brain , DDDDK-tagged CHAT-transfected 293T

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Choline Acetyltransferase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

choline O-acetyltransferase , CHOACTASE , CMS1A , CMS1A2 , CMS6

Background

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Choline Acetyltransferase antibody [N1N3] used in IHC (Frozen sections) (IHC-Fr). GTX113164

GTX113164 IHC-Fr Image

Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein in the amacrine cells by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Choline Acetyltransferase protein stained by Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Choline Acetyltransferase antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113164

GTX113164 ICC/IF Image

Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Choline Acetyltransferase protein stained by Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Choline Acetyltransferase antibody [N1N3] used in Western Blot (WB). GTX113164

GTX113164 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Choline Acetyltransferase antibody [N1N3] used in Western Blot (WB). GTX113164

GTX113164 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Choline Acetyltransferase antibody [N1N3] used in Western Blot (WB). GTX113164

GTX113164 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Choline Acetyltransferase antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113164

GTX113164 IHC-P Image

Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Choline Acetyltransferase stained by Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Choline Acetyltransferase antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113164

GTX113164 IHC-P Image

Choline Acetyltransferase antibody [N1N3] detects Choline Acetyltransferase protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Choline Acetyltransferase stained by Choline Acetyltransferase antibody [N1N3] (GTX113164) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

Choline Acetyltransferase antibody [N1N3] Cat No. GTX113164

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Date : Bernat Nogradi submitted on 22-Mar-2019
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Medulla oblongata region of mouse brain (hypoglossal and vagal nucleus)
Fixation : 4% PFA in PBS, 1 day postfixation
Permeabilization /Antigen retrieval : 0.2% Tritox X-100 in PBS
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169