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Clathrin Light chain antibody [CON.1]

Cat No. GTX11332

Host

Mouse

Clonality

Monoclonal

Clone Name

CON.1

Isotype

IgG2b

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse, Rat, Yeast, Bovine
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2 μg/ml
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

rat brain

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

synthetic peptide (amino acids 23-44 in bovine) common to the N-terminus of mammalian clathrin light chains, LCa and LCb, conjugated to KLH. This region is not involved in heavy chain binding and is completely conserved in all mammals.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

clathrin light chain A , CLTA2

Background

Various cell types express both forms of clathrin LC in different relative levels. LCs are randomly distributed on clathrin triskelion, resulting in four types of clathrin triskelion with different LC composition (3LCa, 2LCa 1LCb, 2LCb-1LCa, 3LCb). In most cells, the LCa form is dominant. In contrast, cells with a regulated secretory pathway contain more LCb than LCa, suggesting that LCb may play a role in the specialized clathrin functions in these cells. Neuronal cells, express splicing variants of both LCa and LCb. A distinguishing feature of the LCb light chain is its ability to be phosphorylated by a casein kinase, at a site which is not present in the LCa sequence.

Database

Research Area

REFERENCE

There are currently no references for Clathrin Light chain antibody [CON.1] (GTX11332). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf