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Claudin 3 antibody

Anti-Claudin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03712
Anti-Claudin 3 antibody used in Flow cytometry (FACS). GTX03712
Anti-Claudin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03712
Anti-Claudin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03712
Anti-Claudin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03712
Anti-Claudin 3 antibody used in Western Blot (WB). GTX03712
Anti-Claudin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03712
Anti-Claudin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03712
Anti-Claudin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03712

Cat. No. GTX03712

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($449)
See all Claudin 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.25μg/ml
ICC/IF 5μg/ml
IHC-P 2-5μg/ml
FACS 1-3μg/1x10⁶ cells
ELISA 0.1-0.5μg/ml
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

No preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human Claudin 3/CLDN3 recombinant protein (Position: A101-V220).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

claudin 3 , C7orf1 , CPE-R2 , CPETR2 , HRVP1 , RVP1

Cellular Localization

Cell membrane

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Claudin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03712

GTX03712 IHC-P Image

IHC-P analysis of human gallbladder adenocarcinoma tissue using GTX03712 Claudin 3 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)
Dilution : 2μg/ml

Anti-Claudin 3 antibody used in Flow cytometry (FACS). GTX03712

GTX03712 FACS Image

FACS analysis of MCF-7 cells using GTX03712 Claudin 3 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Antibody amount : 1μg/1x10⁶ cells

Anti-Claudin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03712

GTX03712 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX03712 Claudin 3 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)
Dilution : 2μg/ml

Anti-Claudin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03712

GTX03712 IHC-P Image

IHC-P analysis of human colonic adenocarcinoma tissue using GTX03712 Claudin 3 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)
Dilution : 2μg/ml

Anti-Claudin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03712

GTX03712 IHC-P Image

IHC-P analysis of human ovarian serous adenocarcinoma tissue using GTX03712 Claudin 3 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)
Dilution : 2μg/ml

Anti-Claudin 3 antibody used in Western Blot (WB). GTX03712

GTX03712 WB Image

WB analysis of various sample lysates using GTX03712 Claudin 3 antibody.
Lane 1 : MCF-7 whole cell lysates
Lane 2 : Caco-2 whole cell lysates
Loading : 30μg per lane
Dilution : 0.25 μg/mL

Anti-Claudin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03712

GTX03712 IHC-P Image

IHC-P analysis of human breast invasive ductal cancer tissue using GTX03712 Claudin 3 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)
Dilution : 2μg/ml

Anti-Claudin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03712

GTX03712 IHC-P Image

IHC-P analysis of human renal clear cell carcinoma tissue using GTX03712 Claudin 3 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)
Dilution : 2μg/ml

Anti-Claudin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03712

GTX03712 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX03712 Claudin 3 antibody.
Green : Primary antibody
Blue : DAPI
Antigen retireval : Enzyme antigen retrieval was performed for 15 mins
Dilution : 5μg/mL

REFERENCE

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REVIEW

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Package List Price ($)
$ 449