Clear Mount mounting medium - general purpose
Procedure:1. Bring the vial to room temperature.2. Rinse slide to be mounted with DISTILLED OR DEIONIZED WATER, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface.3. Turn the vial upside down and open the dropper to remove any air bubbles.4. Apply 3-4 drops of mounting medium directly on top of the specimen and spread out evenly by tilting the slide back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide against a paper towel.5. Let stand at room temperature for about 1 to 2 hours. Or the slides can be heated at 37-40ºC for 40-60 minutes. The slides are ready for visualization under a microscope. For oil immersion lenses, a drop of oil can be placed directly on dried Clear-Mount medium for visualization; the oil can be removed by wiping with Kimwipes.Removal of Coverslip:If the coverslip has to be removed, soak the slide in warm (37ºC) water for 5-10 minutes until the mounting medium is dissolved. Rinse slide with warm water to remove all mounting medium. The slide can be remounted again.
Keep as concentrated solution, aliquot and store at 4ºC. DO NOT FREEZE.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Clear-Mount is a water based mounting medium designed for the permanent mounting of hydrated tissues, which may be damaged with organic solvents. Such samples include cell smears, with peroxidase and alkaline phosphatase chromogens.This mounting medium preserves Fast Red, Aminoethylycarbazole (AEC), NBT/BCIP, INT/BCIP chromogens and is also compatible with counter-stain such as Hematoxylin and Nuclear Fast Red. It is also suitable for chromogens like DAB and DAB with Nickel and Cobalt. It is not compatible with H & E staining.