Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113650 diluted at 1:1000

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 2 antibody (GTX113650) diluted at 1:1000.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Cofilin 2 (muscle)(GTX113650) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 2 antibody (GTX113650) diluted at 1:500.