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Coilin antibody

Orthogonal
Anti-Coilin antibody used in Immunoprecipitation (IP). GTX112570
Anti-Coilin antibody used in IHC (Paraffin sections) (IHC-P). GTX112570
Anti-Coilin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112570
Anti-Coilin antibody used in Western Blot (WB). GTX112570
Anti-Coilin antibody used in Western Blot (WB). GTX112570
Anti-Coilin antibody used in Western Blot (WB). GTX112570

Cat. No. GTX112570

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Coilin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

293T nuclear extract , HepG2 nuclear extract

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Coilin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177315

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coilin , CLN80 , p80-coilin

Cellular Localization

Nucleus

Background

The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Coilin antibody used in Immunoprecipitation (IP). GTX112570

GTX112570 IP Image

Immunoprecipitation of Coilin protein from 293T whole cell extracts using 5 μg of Coilin antibody (GTX112570).
Western blot analysis was performed using Coilin antibody (GTX112570).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Coilin antibody used in IHC (Paraffin sections) (IHC-P). GTX112570

GTX112570 IHC-P Image

Coilin antibody detects Coilin protein at nucleus in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
Coilin antibody (GTX112570) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Coilin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112570

GTX112570 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Coilin(GTX112570) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Anti-Coilin antibody used in Western Blot (WB). GTX112570

GTX112570 WB Image

Orthogonal

Coilin antibody detects Coilin protein by Western blot analysis.
A. 30 μg HepG2 whole cell extract
B. 30 μg HepG2 nuclear extract
7.5 % SDS-PAGE
Coilin antibody (GTX112570) dilution: 1:1000

Anti-Coilin antibody used in Western Blot (WB). GTX112570

GTX112570 WB Image

Orthogonal

Coilin antibody detects Coilin protein by Western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg 293T nuclear extract
7.5 % SDS-PAGE
Coilin antibody (GTX112570) dilution: 1:1000

Anti-Coilin antibody used in Western Blot (WB). GTX112570

GTX112570 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Coilin antibody (GTX112570) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ranjodh Sandhu et al. Proc Natl Acad Sci U S A 2021; 118 (30) The structurally conserved TELR region on shelterin protein TPP1 is essential for telomerase processivity but not recruitment.
Application : ICC/IF
Reactivity : Human
Bogard B et al. Noncoding RNA 2021; 7 (3) Systematic Identification and Functional Validation of New snoRNAs in Human Muscle Progenitors.
Rivera C et al. Epigenetics Chromatin 2021; 14 (1) : 51 Revealing RCOR2 as a regulatory component of nuclear speckles.
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169