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Coilin antibody

Anti-Coilin antibody used in Western Blot (WB). GTX112570
Anti-Coilin antibody used in Western Blot (WB). GTX112570
Anti-Coilin antibody used in IHC (Paraffin sections) (IHC-P). GTX112570
Anti-Coilin antibody used in Immunoprecipitation (IP). GTX112570
Anti-Coilin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112570

Cat No. GTX112570

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

HepG2 , HepG2 nuclear extract , 293T nuclear extract

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Coilin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

coilin,CLN80,p80-coilin

Cellular Localization

Nucleus

Background

The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14. [provided by RefSeq]

Database

DATA IMAGES
Anti-Coilin antibody used in Western Blot (WB). GTX112570

GTX112570 WB Image

Coilin antibody detects Coilin protein by Western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg 293T nuclear extract
7.5 % SDS-PAGE
Coilin antibody (GTX112570) dilution: 1:1000

Anti-Coilin antibody used in Western Blot (WB). GTX112570

GTX112570 WB Image

Coilin antibody detects Coilin protein by Western blot analysis.
A. 30 μg HepG2 whole cell extract
B. 30 μg HepG2 nuclear extract
7.5 % SDS-PAGE
Coilin antibody (GTX112570) dilution: 1:1000

Anti-Coilin antibody used in IHC (Paraffin sections) (IHC-P). GTX112570

GTX112570 IHC-P Image

Coilin antibody detects Coilin protein at nucleus in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
Coilin antibody (GTX112570) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Coilin antibody used in Immunoprecipitation (IP). GTX112570

GTX112570 IP Image

Immunoprecipitation of Coilin protein from 293T whole cell extracts using 5 μg of Coilin antibody (GTX112570).
Western blot analysis was performed using Coilin antibody (GTX112570).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Coilin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112570

GTX112570 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Coilin(GTX112570) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169