Collagen II antibody

Name: Collagen II antibody
Brand: GeneTex
SKU: GTX20300
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Collagen II antibody used in IHC (Paraffin sections) (IHC-P). GTX20300

Anti-Collagen II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20300

Cat. No. GTX20300

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP, Dot, ELISA, IHC, Multiplexing
Reactivity	Human, Rat, Bovine
Package	100 μg ($399)

See all Collagen II products

View product citations for antibody GTX20300 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	Assay dependent
IHC-P	1:50-1:400
IP	1:100
Dot	Assay dependent
ELISA	1:5000-1:50000
IHC	Assay dependent
Multiplexing	Assay dependent

Not tested in other applications.

Calculated MW

142 kDa. ( Note )

Product Note

Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human, Bovine

Immunogen

Collagen Type II from human knee cartilage and bovine nasal cartilage.

Purification

Purified by antigen-affinity chromatography.
Prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. This product has been sterile filtered.

Conjugation

Unconjugated

RRID

AB_374261

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type II alpha 1 chain , ANFH , AOM , COL11A3 , SEDC , STL1

Cellular Localization

Secreted,Extracellular space,Extracellular matrix

Background

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ""Glycine-X-Y"" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

Database

Gene ID: 1280 COL2A1
UniProt: P02458 COL2A1

Research Area

DATA IMAGES

GTX20300 IHC-P Image

GeneTex anti collagen II antibody (GTX20300, 1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100ºC - 20 minutes for antigen retrieval.

GTX20300 ICC/IF Image

The data was published in the journal Oxid Med Cell Longev in 2015.PMID: 26273418

REFERENCE

Application Reference

Wu KC et al. BMC Complement Med Ther 2020; 20 (1) : 364 Aqueous extract of Arctium lappa L. root (burdock) enhances chondrogenesis in human bone marrow-derived mesenchymal stem cells.

Chang YH et al. J Biomed Mater Res A 2020; 108 (10) : 2069-2079 Enhanced chondrogenesis of human umbilical cord mesenchymal stem cells in a gelatin honeycomb scaffold.

Application : WB

Reactivity : Rat

Wang Z et al. Mol Med Rep 2018; 17 (6) : 8237-8243 Icariin promotes stable chondrogenic differentiation of bone marrow mesenchymal stem cells in self-assembling peptide nanofiber hydrogel scaffolds.

Application : IHC

Reactivity : Rat

Maraldi T et al. Oxid Med Cell Longev 2015; 2015 : 101304 Nuclear Nox4 Role in Stemness Power of Human Amniotic Fluid Stem Cells.

Application : ICC/IF

Reactivity : Human

Wang ZC et al. Exp Ther Med 2014; 8 (5) : 1528-1534 Icariin promotes directed chondrogenic differentiation of bone marrow mesenchymal stem cells but not hypertrophy in vitro.

Application : ICC/IF

Reactivity : Rat

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