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Collagen II antibody

Anti-Collagen II antibody used in IHC (Paraffin sections) (IHC-P). GTX20300

Cat No. GTX20300

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ELISA, IHC
Reactivity Human, Mouse, Rat, Sheep, Bovine
Package
100 μg ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:10000
ICC/IF Assay dependent
IHC-P 1:50 - 1:400
IP 1:100
ELISA 1:5000 - 1:50000
IHC Assay dependent
Not tested in other applications.

Calculated MW

142 kDa. ( Note )

Positive Control

* chondrocyte tissue ,

Specificity/Sensitivity

Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards.  Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.   This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens.  Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
PROPERTIES

Form

Liquid

Buffer

0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Collagen Type II from human knee cartilage and bovine nasal cartilage.

Purification

Purified by antigen-affinity chromatography.
Prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. This product has been sterile filtered.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

collagen type II alpha 1 chain , ANFH , AOM , COL11A3 , SEDC , STL1

Background

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ""Glycine-X-Y"" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

Database

Research Area

DATA IMAGES
Anti-Collagen II antibody used in IHC (Paraffin sections) (IHC-P). GTX20300

GTX20300 IHC-P Image

GeneTex anti collagen II antibody (GTX20300, 1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.

REFERENCE
REVIEW

Collagen II antibody Cat No. GTX20300

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 20-Feb-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : chondrocyte tissue
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Package List Price ($)
$ 319