GeneTex
United States (US)

Collagen III antibody

GTX27778_20160330_WB_18121410_330.jpg
GTX27778_20160330_IHC-P_18121410_803.jpg

Cat No. GTX27778

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr, IP, ELISA
Reactivity Human, Bovine
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:10000
IHC-P 1:50 - 1:200
IHC-Fr Assay dependent
IP 1:100
ELISA 1:5000 - 1:50000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Specificity/Sensitivity

Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards.  Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.  This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V or VI collagens.  Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
PROPERTIES

Form

Liquid

Buffer

0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0mg/ml(Please refer to the vial label for the specific concentration.)

Immunogen

Collagen Type III from human and bovine placenta

Purification

Purified by antigen-affinity chromatography.
Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

COL3A1 Antibody , EDS4A Antibody

Cellular Localization

Extracellular matrix

Background

These antibodies are well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix, which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Database

DATA IMAGES
GTX27778_20160330_WB_18121410_330.jpg

GTX27778 WB Image

Western Blot of Rabbit Anti-Collagen III Antibody (GTX27778) Load: 100 ng Human Collagen III. Primary antibody: Collagen III Antibody at 1:1000 o/n at 4°C. Secondary antibody: DyLight™ 649 Goat anti-rabbit at 1:20,000 for 30 min at RT Block for 30 min at RT. Predicted/Observed size: 138 kDa, 138 kDa.

GTX27778_20160330_IHC-P_18121410_803.jpg

GTX27778 IHC-P Image

GeneTex anti collagen III antibody (GTX27778, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.

REFERENCE
Package List Price ($)
$ 289