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Collagen IV antibody

Anti-Collagen IV antibody used in IHC (Paraffin sections) (IHC-P). GTX26586

Cat No. GTX26586

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP, ELISA
Reactivity Human, Bovine
Package
100 μg ($289)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:10000
IHC-P 1:50 - 1:200
IP 1:100
ELISA 1:5000 - 1:50000
Not tested in other applications.

Specificity/Sensitivity

Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards.  Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.  This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens.  Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
PROPERTIES

Form

Liquid

Buffer

0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Collagen Type IV from human and bovine placenta

Purification

Purified by antigen-affinity chromatography.
Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HANAC Antibody , COL4A1 Antibody , ICH Antibody , POREN1 Antibody , ARRESTEN Antibody

Cellular Localization

Cell surface (Potential).

Background

This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix, which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Research Area

DATA IMAGES
Anti-Collagen IV antibody used in IHC (Paraffin sections) (IHC-P). GTX26586

GTX26586 IHC-P Image

GeneTex anti collagen IV antibody (GTX26586, 1:400, 45 min RT) showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.

REFERENCE
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Package List Price ($)
$ 289