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ConA Lectin (FITC)

Cat. No. GTX01505

Application

ICC/IF

Species

Jack Bean (Canavalia ensiformis)
Package
1 mg ($89)
PRODUCT

Summary

Concanavalin A is a lectin protein (MW 104kDa), homotetramer 26 kDa; originally extracted from the jack-bean, Canavalia ensiformis. It binds specifically to certain structures found in various sugars α-mannosyl and α-glucosyl residues in glycoproteins. It was the first lectin to be available on a commercial basis and is widely used in biology and biochemistry to characterize glycoproteins and other sugar-containing entities. It is also used to purify macromolecules in lectin affinity chromatography. Concanavalin A interacts with diverse receptors containing mannose carbohydrates (serum and membrane glycoproteins).ConA agglutinate strongly erythrocytes without being blood group specific. Normal cell re agglutinated after trypsinisation. ConA is not also a lymphocyte mitogen and reacts with many bacteria, like E. coli Dictyostelium discoideum et B. substilis, but also widely believed to be involved in the interaction between alpha-mannosyl oligosaccharides on the surface of the HIV virus and the human T cell lymphocyte.

APPLICATION

Product Note

alpha-linked mannose and glucose

PROPERTIES

Form

Liquid

Buffer

10mM Bicarbonate, 150mM NaCl, 0.1mM CaCl₂, 0.01mM MgCl₂ (Con A has an Isoelectric point of about pH4.5-5.5, requires calcium or manganese ions at each of its four saccharide binding sites. These ions should be used in buffer. DO NOT USE Phosphate buffer for dilution of this lectin as it will decrease the activity of lectin).

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

5 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

Native Protein

Expression System

Native Protein

Purification


Purified from Canavalia ensiformis

Conjugation

Fluorescein isothiocyanate (FITC) Wavelength
Absorption : 490 nm ; Emission : 520 nm. Ratio : 1.0-1.4 molecules FITC (isomer 1) per Lectin molecule.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Concanavalin A , Concanavalin A , Canavalia ensiformis , Canavalia ensiformis lectin , ConA lectin , Canavalia ensiformis , ConA , Concanavalin A lectin , Con A lectin

Background

Concanavalin A is a lectin protein (MW 104kDa), homotetramer 26 kDa; originally extracted from the jack-bean, Canavalia ensiformis. It binds specifically to certain structures found in various sugars α-mannosyl and α-glucosyl residues in glycoproteins. It was the first lectin to be available on a commercial basis and is widely used in biology and biochemistry to characterize glycoproteins and other sugar-containing entities. It is also used to purify macromolecules in lectin affinity chromatography. Concanavalin A interacts with diverse receptors containing mannose carbohydrates (serum and membrane glycoproteins).ConA agglutinate strongly erythrocytes without being blood group specific. Normal cell re agglutinated after trypsinisation. ConA is a also a lymphocyte mitogen.ConA reacts with many bacteria, like E. coli Dictyostelium discoideum et B. substilis It is also widely believed to be involved in the interaction between alpha-mannosyl oligosaccharides on the surface of the HIV virus and the human T cell lymphocyte. Inhibiting/Eluting sugars: 200 α-mM α-methyl mannoside / 200 mM α-methyl glucoside mixture.
Carbohydrate-Binding Specificity of Con A: (Manα1,2Manα1,2Man > Manα1,2Man > α-Man > α-Glc > αGlcNAc

Research Area

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 89