APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Rabbit, Bovine, Dog, Chicken, Guinea pig, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.65 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Intracellular domain of human Connexin 43. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886808
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
gap junction protein alpha 1 , AVSD3 , CMDR , CX43 , EKVP , EKVP3 , GJAL , HLHS1 , HSS , ODDD , PPKCA
Cellular Localization
Cell membrane,Endoplasmic reticulum
Background
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. [provided by RefSeq, May 2014]
Database
Research Area
DATA IMAGES
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GTX133022 IHC-P Image
Connexin 43 antibody detects Connexin 43 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.Connexin 43 stained by Connexin 43 antibody (GTX133022) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133022 ICC/IF Image
Connexin 43 antibody detects Connexin 43 protein by immunofluorescent analysis.Sample: U87-MG cells were fixed in ice-cold MeOH for 5 min.Green: Connexin 43 stained by Connexin 43 antibody (GTX133022) diluted at 1:500.Red: phalloidin, a cytoskeleton marker diluted at 1:200.Blue: Hoechst 33342 staining.
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GTX133022 WB Image
Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Connexin 43 antibody (GTX133022) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133022 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Connexin 43 antibody (GTX133022) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133022 ICC/IF Image
Connexin 43 antibody detects Connexin 43 protein at cell membrane by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Connexin 43 stained by Connexin 43 antibody (GTX133022) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm.
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REFERENCE
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