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Cortactin antibody [N1], N-term

KO/KD
Anti-Cortactin antibody [N1], N-term used in Western Blot (WB). GTX100253
Anti-Cortactin antibody [N1], N-term used in Western Blot (WB). GTX100253
Anti-Cortactin antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100253
Anti-Cortactin antibody [N1], N-term used in Western Blot (WB). GTX100253
Anti-Cortactin antibody [N1], N-term used in Western Blot (WB). GTX100253
Anti-Cortactin antibody [N1], N-term used in Western Blot (WB). GTX100253
Anti-Cortactin antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100253
Anti-Cortactin antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100253

Cat. No. GTX100253

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Cortactin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

NIH-3T3 , JC , PC-12 , mouse brain , rat brain , HeLa

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Cortactin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240648

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cortactin , EMS1

Cellular Localization

Cytoplasm , cytoskeleton , Cell projection , lamellipodium , ruffle

Background

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cortactin antibody [N1], N-term used in Western Blot (WB). GTX100253

GTX100253 WB Image

KO/KD

Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term (GTX100253) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cortactin antibody [N1], N-term used in Western Blot (WB). GTX100253

GTX100253 WB Image

Sample (30 ug of whole cell lysate)
A: PC-12
7.5% SDS PAGE
GTX100253 diluted at 1:3000

Anti-Cortactin antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100253

GTX100253 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Cortactin(GTX100253) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cortactin antibody [N1], N-term used in Western Blot (WB). GTX100253

GTX100253 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX100253 diluted at 1:1000

Anti-Cortactin antibody [N1], N-term used in Western Blot (WB). GTX100253

GTX100253 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term (GTX100253) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cortactin antibody [N1], N-term used in Western Blot (WB). GTX100253

GTX100253 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term (GTX100253) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Multiple bands were observed 65 kDa, 70 kDa , and 74 kDa. It is possibly due to alternative splicing.
Reference:PMID: 27903975

Anti-Cortactin antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100253

GTX100253 ICC/IF Image

Cortactin antibody [N1], N-term detects Cortactin protein at cytoplasm and cell membrane by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cortactin stained by Cortactin antibody [N1], N-term (GTX100253) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-Cortactin antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX100253

GTX100253 IHC-P Image

Cortactin antibody [N1], N-term detects Cortactin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Cortactin stained by Cortactin antibody [N1], N-term (GTX100253) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Tonucci FM et al. Cancer Lett 2019; 461 : 65-77 Identification of a CIP4 PKA phosphorylation site involved in the regulation of cancer cell invasiveness and metastasis.
Application : ICC/IF
Reactivity : Human
Stakaityt? G et al. J Virol 2017; (Epub) Merkel cell polyomavirus small T antigen drives cell motility via Rho-GTPase-induced filopodia formation.
Application : WB
Reactivity : Human
Yun-Hong Y et al. Mol Cell Proteomics 2011; 10 (10) : M110.007138 A study of the spatial protein organization of the postsynaptic density isolated from porcine cerebral cortex and cerebellum.
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169