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Cortactin antibody [N1C1]

Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680
Anti-Cortactin antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113680
Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680
Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680
Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680
Anti-Cortactin antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113680
Anti-Cortactin antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113680

Cat No. GTX113680

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

HeLa , Neuro 2A , C8D30 , NIH-3T3 , C2C12 , PC-12 , Rat2 , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cortactin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618119

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cortactin , EMS1

Cellular Localization

Cytoplasm , cytoskeleton , Cell projection , lamellipodium , ruffle

Background

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680

GTX113680 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1] (GTX113680) diluted at 1:10000.

Anti-Cortactin antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113680

GTX113680 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Cortactin(GTX113680) antibody at 1:200 dilution.

Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680

GTX113680 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1] (GTX113680) diluted at 1:10000.

Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680

GTX113680 WB Image

Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1] (GTX113680) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680

GTX113680 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1] (GTX113680) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Multiple bands were observed 65 kDa, 70 kDa , and 74 kDa. It is possibly due to alternative splicing.
Reference:PMID: 27903975

Anti-Cortactin antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113680

GTX113680 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Cortactin(GTX113680) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cortactin antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113680

GTX113680 IHC-P Image

Cortactin antibody [N1C1] detects Cortactin protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Cortactin antibody [N1C1] (GTX113680) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for Cortactin antibody [N1C1] (GTX113680). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Cortactin antibody [N1C1] (GTX113680). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169