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Cyclin D1 antibody [DCS-6]

Anti-Cyclin D1 antibody [DCS-6] used in Flow cytometry (FACS). GTX34618
Anti-Cyclin D1 antibody [DCS-6] used in IHC (Paraffin sections) (IHC-P). GTX34618
Anti-Cyclin D1 antibody [DCS-6] used in Western Blot (WB). GTX34618

Cat No. GTX34618

Host

Mouse

Clonality

Monoclonal

Clone Name

DCS-6

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT
FACS Assay dependent

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Specificity/Sensitivity

Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). About 40% of breast carcinomas are positive for Cyclin D1. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Human full length recombinant cyclin D1 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cyclin D1 , Bcl1 , D11S287E , Prad1 , U21B31 , Ccnd1

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cyclin D1 antibody [DCS-6] used in Flow cytometry (FACS). GTX34618

GTX34618 FACS Image

Flow Cytometry of human Cyclin D1 on Jurkat Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Monoclonal Antibody (DCS-6).

Anti-Cyclin D1 antibody [DCS-6] used in IHC (Paraffin sections) (IHC-P). GTX34618

GTX34618 IHC-P Image

Formalin-fixed, paraffin-embedded human Mantle Cell Lymphoma stained with Cyclin D1 Monoclonal Antibody (DCS-6).

Anti-Cyclin D1 antibody [DCS-6] used in Western Blot (WB). GTX34618

GTX34618 WB Image

Western Blot of Cyclin D1 in 1) C2C12, 2) HepG2, & 3) NIH3T3 Lysate using Cyclin D1 Monoclonal Antibody (DCS-6).

REFERENCE

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Package List Price ($)
$ 429