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Cyclin D1 antibody [DCS-6]

Anti-Cyclin D1 antibody [DCS-6] used in Flow cytometry (FACS). GTX34618
Anti-Cyclin D1 antibody [DCS-6] used in IHC (Paraffin sections) (IHC-P). GTX34618
Anti-Cyclin D1 antibody [DCS-6] used in Western Blot (WB). GTX34618

Cat. No. GTX34618

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 DCS-6

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μg ($529)
See all Cyclin D1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human full length recombinant cyclin D1 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

RRID

AB_2887739

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL1 , CCND1 , D11S287E , PRAD1 , U21B31 , cyclin D1 , Cyclin D1

Cellular Localization

Nucleus,Cytoplasm,Membrane

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Cyclin D1 antibody [DCS-6] used in Flow cytometry (FACS). GTX34618

GTX34618 FACS Image

FACS analysis of Jurkat cells using GTX34618 Cyclin D1 antibody [DCS-6].
Black : Blank
Green : Isotype Control
Red : Primary antibody

Anti-Cyclin D1 antibody [DCS-6] used in IHC (Paraffin sections) (IHC-P). GTX34618

GTX34618 IHC-P Image

IHC-P analysis of human mantle cell lymphoma tissue using GTX34618 Cyclin D1 antibody [DCS-6].

Anti-Cyclin D1 antibody [DCS-6] used in Western Blot (WB). GTX34618

GTX34618 WB Image

WB analysis of (1) C2C12, (2) HepG2, & (3) NIH3T3 cell lysates using GTX34618 Cyclin D1 antibody [DCS-6].

REFERENCE

Application Reference

Wang DW et al. Eur. Rev. Med. Pharmacol. Sci. 2018; 22 : 1241-1248 MiR-16 inhibits pituitary adenoma cell proliferation via the suppression of ERK/MAPK signal pathway.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529