Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cyclin D2 antibody (GTX133418) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cyclin D2 antibody detects Cyclin D2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cyclin D2 protein stained by Cyclin D2 antibody (GTX133418) diluted at 1:500.
Blue: Hoechst 33342 staining.

Cyclin D2 antibody detects Cyclin D2 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Cyclin D2 antibody (GTX133418) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min