GeneTex
United States (US)

Cyclin E1 antibody

Orthogonal
GTX103045_40373_WB_19011506_169.jpg
GTX103045_40373_20170929_WB_19011506_899.jpg
GTX103045_40373_20170929_WB_Hydroxyurea_19011506_616.jpg
GTX103045_40373_IFA_19011506_310.jpg

Cat No. GTX103045

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

U87-MG , MCF-7 , MDA-MB-231 , HCT-116 , HCT-116 (1 mM Hydroxyurea treatment for 24hr)

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Cyclin E1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CCNE antibody, CCNE1 antibody, cyclin Es antibody, G1/S-specific cyclin-E1 antibody, cyclin Et antibody, cyclin E1 antibody

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103045_40373_WB_19011506_169.jpg

GTX103045 WB Image

Sample (30 µg of whole cell lysate)
A: U87-MG
10% SDS PAGE
GTX103045 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103045_40373_20170929_WB_19011506_899.jpg

GTX103045 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin E1 antibody (GTX103045) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed M.W. is based on the publication: PMID: 22433433

GTX103045_40373_20170929_WB_Hydroxyurea_19011506_616.jpg

GTX103045 WB Image

Orthogonal

Untreated (–) and treated (+) HCT-116 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin E1 antibody (GTX103045) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103045_40373_IFA_19011506_310.jpg

GTX103045 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Cyclin E1(GTX103045) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169