APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| Recommended starting dilution is 1:1000. |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS (pH7.4), 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT3G6 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Cyclin H protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
cyclin H , CAK , CCNH , CycH , p34 , p37
Cellular Localization
Nucleus
Background
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Nov 2010]
Database
Research Area
DATA IMAGES
![Anti-Cyclin H antibody [AT3G6] used in Western Blot (WB). GTX57623](/templates/default/js/lightbox/images/loading.gif "Anti-Cyclin H antibody [AT3G6] used in Western Blot (WB). GTX57623")
|
GTX57623 WB Image
WB analysis of Recombinant Human Cyclin H protein using GTX57623 Cyclin H antibody.
Loading : 25 ng
Dilution : 1:1000
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GTX57623 WB Image
WB analysis of various samples using GTX57623 Cyclin H antibody.
Lane 1 : HepG2 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : Ramos whole cell lysate
Lane 4 : Bal/3T3 whole cell lysate
Loading : 40 μg
Dilution : 1:1000
|
REFERENCE
There are currently no references for Cyclin H antibody [AT3G6] (GTX57623). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cyclin H antibody [AT3G6] (GTX57623). Be the first to share your experience with this product.
![Anti-Cyclin H antibody [AT3G6] used in Western Blot (WB). GTX57623](/upload/website/prouct_img/small/GTX57623/GTX57623_20200204_WB1_20020321_945.jpg "Anti-Cyclin H antibody [AT3G6] used in Western Blot (WB). GTX57623")
![Anti-Cyclin H antibody [AT3G6] used in Western Blot (WB). GTX57623](/upload/website/prouct_img/small/GTX57623/GTX57623_20200204_WB2_20020321_339.jpg "Anti-Cyclin H antibody [AT3G6] used in Western Blot (WB). GTX57623")
![Anti-Cyclin H antibody [AT3G6] used in Western Blot (WB). GTX57623](/upload/website/prouct_img/normal/GTX57623/GTX57623_20200204_WB1_20020321_945.jpg "Anti-Cyclin H antibody [AT3G6] used in Western Blot (WB). GTX57623")
![Anti-Cyclin H antibody [AT3G6] used in Western Blot (WB). GTX57623](/upload/website/prouct_img/normal/GTX57623/GTX57623_20200204_WB2_20020321_339.jpg "Anti-Cyclin H antibody [AT3G6] used in Western Blot (WB). GTX57623")