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Cyclin H antibody [AT3G6]

Anti-Cyclin H antibody [AT3G6] used in Western Blot (WB). GTX57623
Anti-Cyclin H antibody [AT3G6] used in Western Blot (WB). GTX57623

Cat. No. GTX57623

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT3G6

Isotype

 IgG2b

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Cyclin H products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Recommended starting dilution is 1:1000.
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT3G6 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Cyclin H protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin H , CAK , CCNH , CycH , p34 , p37

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-Cyclin H antibody [AT3G6] used in Western Blot (WB). GTX57623

GTX57623 WB Image

WB analysis of Recombinant Human Cyclin H protein using GTX57623 Cyclin H antibody.
Loading : 25 ng
Dilution : 1:1000

Anti-Cyclin H antibody [AT3G6] used in Western Blot (WB). GTX57623

GTX57623 WB Image

WB analysis of various samples using GTX57623 Cyclin H antibody.
Lane 1 : HepG2 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : Ramos whole cell lysate
Lane 4 : Bal/3T3 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399