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Cyclin H antibody [SN20-48]

Recombinant antibody
Anti-Cyclin H antibody [SN20-48] used in Western Blot (WB). GTX01109
Anti-Cyclin H antibody [SN20-48] used in Western Blot (WB). GTX01109
Anti-Cyclin H antibody [SN20-48] used in Western Blot (WB). GTX01109
Anti-Cyclin H antibody [SN20-48] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01109
Anti-Cyclin H antibody [SN20-48] used in IHC (Paraffin sections) (IHC-P). GTX01109
Anti-Cyclin H antibody [SN20-48] used in Flow cytometry (FACS). GTX01109
Anti-Cyclin H antibody [SN20-48] used in IHC (Paraffin sections) (IHC-P). GTX01109
Anti-Cyclin H antibody [SN20-48] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01109
Anti-Cyclin H antibody [SN20-48] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01109

Cat No. GTX01109

Host

Rabbit

Clonality

Monoclonal

Clone Name

SN20-48

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin H , CAK , CCNH , CycH , p34 , p37

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-Cyclin H antibody [SN20-48] used in Western Blot (WB). GTX01109

GTX01109 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin H antibody [SN20-48] (GTX01109) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin H antibody [SN20-48] used in Western Blot (WB). GTX01109

GTX01109 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin H antibody [SN20-48] (GTX01109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin H antibody [SN20-48] used in Western Blot (WB). GTX01109

GTX01109 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin H antibody [SN20-48] (GTX01109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cyclin H antibody [SN20-48] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01109

GTX01109 ICC/IF Image

ICC/IF analysis of PFA-fixed MCF-7 cells using GTX01109 Cyclin H antibody [SN20-48].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-Cyclin H antibody [SN20-48] used in IHC (Paraffin sections) (IHC-P). GTX01109

GTX01109 IHC-P Image

IHC-P analysis of human colon cancer tissue section using GTX01109 Cyclin H antibody [SN20-48].

Anti-Cyclin H antibody [SN20-48] used in Flow cytometry (FACS). GTX01109

GTX01109 FACS Image

FACS analysis of HeLa cells using GTX01109 Cyclin H antibody [SN20-48].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50

Anti-Cyclin H antibody [SN20-48] used in IHC (Paraffin sections) (IHC-P). GTX01109

GTX01109 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX01109 Cyclin H antibody [SN20-48].

Anti-Cyclin H antibody [SN20-48] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01109

GTX01109 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX01109 Cyclin H antibody [SN20-48].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-Cyclin H antibody [SN20-48] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01109

GTX01109 ICC/IF Image

ICC/IF analysis of PFA-fixed PC-3M cells using GTX01109 Cyclin H antibody [SN20-48].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

REFERENCE

There are currently no references for Cyclin H antibody [SN20-48] (GTX01109). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Cyclin H antibody [SN20-48] (GTX01109). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369