Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CysLT1 Receptor antibody [N1], N-term (GTX108112) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using CysLT 1 receptor(GTX108112) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min