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Cystatin B antibody

Anti-Cystatin B antibody used in IHC (Paraffin sections) (IHC-P). GTX101458
Anti-Cystatin B antibody used in IHC (Paraffin sections) (IHC-P). GTX101458
Anti-Cystatin B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101458

Cat. No. GTX101458

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Cystatin B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Predict Reactivity

Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

6.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cystatin B. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2036718

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cystatin B , CPI-B , CST6 , EPM1 , EPM1A , PME , STFB , ULD

Cellular Localization

Cytoplasm , Nucleus

Background

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cystatin B antibody used in IHC (Paraffin sections) (IHC-P). GTX101458

GTX101458 IHC-P Image

Cystatin B antibody detects Cystatin B protein at cytoplasm and nucleus human on tonsil by immunohistochemical analysis.
Sample: Paraffin-embedded tonsil.
Cystatin B antibody (GTX101458) dilution: 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cystatin B antibody used in IHC (Paraffin sections) (IHC-P). GTX101458

GTX101458 IHC-P Image

Cystatin B antibody detects Cystatin B protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Cystatin B antibody (GTX101458) diluted at 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cystatin B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101458

GTX101458 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using Cystatin B(GTX101458) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169