APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 0.5-1ug/ml |
| 0.1-0.2ug/ml for 30 minutes at RT |
|
Note :
IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
|
Not tested in other applications.
Calculated MW
Specificity/Sensitivity
This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
PROPERTIES
Form
Liquid
Buffer
10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml
Antigen Species
Human
Immunogen
Skin extract of a human Psoriasis patient
Purification
Ab purified from Bioreactor Concentrate by Protein A/G
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Keratin 10 , Bcie , Bie , Ck10 , Ehk , K10 , Kpp , Krt10
Background
This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Cytokeratin 10 antibody [SPM261] used in IHC (Paraffin sections) (IHC-P). GTX34624](/upload/website/prouct_img/middle/GTX34624/GTX34624_20170712_IHC-P_18121410_865.jpg "Anti-Cytokeratin 10 antibody [SPM261] used in IHC (Paraffin sections) (IHC-P). GTX34624")
|
GTX34624 IHC-P Image
Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Cytokeratin 10 Monoclonal Antibody (SPM261).
|
REFERENCE
There are currently no references for Cytokeratin 10 antibody [SPM261] (GTX34624). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cytokeratin 10 antibody [SPM261] (GTX34624). Be the first to share your experience with this product.
![Anti-Cytokeratin 10 antibody [SPM261] used in IHC (Paraffin sections) (IHC-P). GTX34624](/upload/website/prouct_img/small/GTX34624/GTX34624_20170712_IHC-P_18121410_865.jpg "Anti-Cytokeratin 10 antibody [SPM261] used in IHC (Paraffin sections) (IHC-P). GTX34624")
![Anti-Cytokeratin 10 antibody [SPM261] used in IHC (Paraffin sections) (IHC-P). GTX34624](/upload/website/prouct_img/normal/GTX34624/GTX34624_20170712_IHC-P_18121410_865.jpg "Anti-Cytokeratin 10 antibody [SPM261] used in IHC (Paraffin sections) (IHC-P). GTX34624")
