GeneTex
United States (US)

Cytokeratin 18 antibody [DC10]

GTX34647_20170712_WB_18121410_379.jpg
GTX34647_20170712_IHC-P_18121410_925.jpg

Cat No. GTX34647

Host Mouse
Clonality Monoclonal
Clone Name DC10
Isotype IgG1
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

WB:0.5-1ug/ml, IHC-P:0.5-1ug/ml for 30 min at RT.

Calculated MW

48 kDa. ( Note )

Specificity/Sensitivity

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

PMC-42 human breast carcinoma cells

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Keratin 18,Ck-18,Cyk18,K18,Krt18

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX34647_20170712_WB_18121410_379.jpg

GTX34647 WB Image

Western Blot 1) HeLa and 2) A431 Lysate using Cytokeratin 18 Monoclonal Antibody (DC10).

GTX34647_20170712_IHC-P_18121410_925.jpg

GTX34647 IHC-P Image

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (DC10).

Package List Price ($)
$ 329