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Cytokeratin 18 antibody [N1N3]

KO/KD
Anti-Cytokeratin 18 antibody [N1N3] used in Western Blot (WB). GTX112977
Anti-Cytokeratin 18 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112977
Anti-Cytokeratin 18 antibody [N1N3] used in Western Blot (WB). GTX112977
Anti-Cytokeratin 18 antibody [N1N3] used in Western Blot (WB). GTX112977
Anti-Cytokeratin 18 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112977

Cat. No. GTX112977

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Cytokeratin 18 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

A549 , H1299 , HeLa

Predict Reactivity

Mouse, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 18. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10719880

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 18 , CK-18 , CYK18 , K18

Cellular Localization

Cytoplasm , perinuclear region

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 18 antibody [N1N3] used in Western Blot (WB). GTX112977

GTX112977 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N1N3] (GTX112977) diluted at 1:15000.

Anti-Cytokeratin 18 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112977

GTX112977 IHC-P Image

Immunohistochemical analysis of paraffin-embedded hepatoma, using Cytokeratin 18 antibody(GTX112977) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 18 antibody [N1N3] used in Western Blot (WB). GTX112977

GTX112977 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N1N3] (GTX112977) diluted at 1:5000.

Anti-Cytokeratin 18 antibody [N1N3] used in Western Blot (WB). GTX112977

GTX112977 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N1N3] (GTX112977) diluted at 1:5000.

Anti-Cytokeratin 18 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112977

GTX112977 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 18(GTX112977) antibody at 1:200 dilution.

REFERENCE

Application Reference

Salina ACG et al. Elife 2022; 11 Efferocytosis of SARS-CoV-2-infected dying cells impairs macrophage anti-inflammatory functions and clearance of apoptotic cells.
Huang HJ et al. Mol Biosyst 2014; (Epub) Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169