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Cytokeratin 18 antibody [N2C2], Internal

Anti-Cytokeratin 18  antibody [N2C2], Internal used in Western Blot (WB). GTX105624
Anti-Cytokeratin 18  antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105624
Anti-Cytokeratin 18  antibody [N2C2], Internal used in Western Blot (WB). GTX105624
Anti-Cytokeratin 18  antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105624
Anti-Cytokeratin 18  antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105624

Cat No. GTX105624

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa , H1299 , HepG2

Predict Reactivity

Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 18. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

keratin 18 , CK-18 , CYK18 , K18

Cellular Localization

Cytoplasm , perinuclear region

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Cytokeratin 18  antibody [N2C2], Internal used in Western Blot (WB). GTX105624

GTX105624 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N2C2], Internal (GTX105624) diluted at 1:2000.

Anti-Cytokeratin 18  antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105624

GTX105624 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human endo mitral ovarian cancer, using KRT18(GTX105624) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cytokeratin 18  antibody [N2C2], Internal used in Western Blot (WB). GTX105624

GTX105624 WB Image

Sample(30 μg of whole cell lysate)
A:H1299
B:Hep G2(GTX27900)
7.5% SDS PAGE
GTX105624 diluted at 1:3000

Anti-Cytokeratin 18  antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105624

GTX105624 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 18 (GTX105624) antibody at 1:200 dilution.

Anti-Cytokeratin 18  antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105624

GTX105624 ICC/IF Image

Cytokeratin 18 antibody [N2C2], Internal detects Cytokeratin 18 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Cytokeratin 18 protein stained by Cytokeratin 18 antibody [N2C2], Internal (GTX105624) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169