Cytokeratin 18 antibody [N2C2], Internal

Name: Cytokeratin 18 antibody [N2C2], Internal
Brand: GeneTex
SKU: GTX105624
Price: 399.00 USD

Anti-Cytokeratin 18 antibody [N2C2], Internal used in Western Blot (WB). GTX105624

Anti-Cytokeratin 18 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105624

Anti-Cytokeratin 18 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105624

Cat. No. GTX105624

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Dog
Package	100 μl ($399), 25 μl ($169)

See all Cytokeratin 18 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	Assay dependent

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

H1299 , HepG2 , HeLa

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 18. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950645

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 18 , CK-18 , CYK18 , K18

Cellular Localization

Cytoplasm , perinuclear region

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Gene ID: 3875 KRT18
UniProt: P05783 KRT18

Research Area

DATA IMAGES

GTX105624 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N2C2], Internal (GTX105624) diluted at 1:2000.

GTX105624 ICC/IF Image

Cytokeratin 18 antibody [N2C2], Internal detects Cytokeratin 18 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Cytokeratin 18 protein stained by Cytokeratin 18 antibody [N2C2], Internal (GTX105624) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX105624 WB Image

Sample(30 μg of whole cell lysate)
A:H1299
B:Hep G2(GTX27900)
7.5% SDS PAGE
GTX105624 diluted at 1:3000

GTX105624 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 18 (GTX105624) antibody at 1:200 dilution.

GTX105624 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human endo mitral ovarian cancer, using KRT18(GTX105624) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105624 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cytoskeleton by immunohistochemical analysis.
Sample: Paraffin-embedded dog malignant carcinoma.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX105624 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog colon.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX105624 ICC/IF Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500.

GTX105624 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog intestine.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX105624 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog kidney.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX105624 IHC-P Image

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog liver.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX105624 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody (GTX105624) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Wuputra K et al. Int J Mol Sci 2022; 23 (14) FOXM1-CD44 Signaling Is Critical for the Acquisition of Regorafenib Resistance in Human Liver Cancer Cells.

Yu HI et al. J Pharm Biomed Anal 2018; 160 : 344-350 Proteomic analysis of evodiamine-induced cytotoxicity in thyroid cancer cells.

Application : WB

Reactivity : Human

Luni C et al. Nat Methods 2016; 13 (5) : 446-52 High-efficiency cellular reprogramming with microfluidics.

Application : ICC/IF

Reactivity : Human

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.

Application : IP

Reactivity : Human

Xing C et al. Neoplasia 2014; 16 (11) : 883-99 Klf5 deletion promotes Pten deletion-initiated luminal-type mouse prostate tumors through multiple oncogenic signaling pathways.

Application : IHC-P

Reactivity : Mouse

Submit a Reference

REVIEW

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Application Reference

Wuputra K et al. Int J Mol Sci 2022; 23 (14) FOXM1-CD44 Signaling Is Critical for the Acquisition of Regorafenib Resistance in Human Liver Cancer Cells.

Yu HI et al. J Pharm Biomed Anal 2018; 160:344-350 Proteomic analysis of evodiamine-induced cytotoxicity in thyroid cancer cells.

Application : WB

Reactivity : Human

Luni C et al. Nat Methods 2016; 13 (5):446-52 High-efficiency cellular reprogramming with microfluidics.

Application : ICC/IF

Reactivity : Human

Sundararaman B et al. Mol Cell 2016; 61 (6):903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.

Application : IP

Reactivity : Human

Xing C et al. Neoplasia 2014; 16 (11):883-99 Klf5 deletion promotes Pten deletion-initiated luminal-type mouse prostate tumors through multiple oncogenic signaling pathways.

Application : IHC-P

Reactivity : Mouse

SDS
Glycerol.pdf
Thimerosal.pdf

Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty

Cytokeratin 18 antibody [N2C2], Internal

Cat. No. GTX105624

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX105624 WB Image

GTX105624 ICC/IF Image

GTX105624 WB Image

GTX105624 ICC/IF Image

GTX105624 IHC-P Image

GTX105624 IHC-P Image

GTX105624 IHC-P Image

GTX105624 ICC/IF Image

GTX105624 IHC-P Image

GTX105624 IHC-P Image

GTX105624 IHC-P Image

GTX105624 WB Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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