Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N2C2], Internal (GTX105624) diluted at 1:2000.

Immunohistochemical analysis of paraffin-embedded human endo mitral ovarian cancer, using KRT18(GTX105624) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 18 (GTX105624) antibody at 1:200 dilution.

Sample(30 μg of whole cell lysate)
A:H1299
B:Hep G2(GTX27900)
7.5% SDS PAGE
GTX105624 diluted at 1:3000

Cytokeratin 18 antibody [N2C2], Internal detects Cytokeratin 18 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Cytokeratin 18 protein stained by Cytokeratin 18 antibody [N2C2], Internal (GTX105624) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody (GTX105624) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog liver.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog kidney.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog intestine.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500.

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog colon.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Cytokeratin 18 antibody detects Cytokeratin 18 protein at cytoskeleton by immunohistochemical analysis.
Sample: Paraffin-embedded dog malignant carcinoma.
Cytokeratin 18 stained by Cytokeratin 18 antibody (GTX105624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min