GeneTex
United States (US)

Cytokeratin 5 + 14 antibody [16.4]

Cat No. GTX16570

Host Mouse
Clonality Monoclonal
Clone Name 16.4
Isotype IgG2a
Application WB, IHC-Fr, IHC-F
Reactivity Human
APPLICATION

Application Note

IHC: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects bands of approximately 58 and 50 kDa corresponding to cytokeratins 5 and 14 respectively. Optimal dilutions/concentrations should be determined by the end user.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.08% Sodium Azide. Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Cat

Immunogen

Tissue / cell preparation (cytokeratin enriched extract of feline tongue epithelium).

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Basal layer

Background

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis.Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells.Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Belonging to the type A (acidic) subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas.
Package List Price ($)
$ 329