*Optimal dilutions/concentrations should be determined by the researcher.
|1-2μg/ml for 30 minutes at RT
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.
This antibody recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs.
10mM PBS, 0.05% BSA
0.05% Sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Semi-Purified cytokeratin preparation.
Protein A/G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
CK7 , K2C7 , K7 , KRT7 , SCL , keratin 7 , Cytokeratin 7
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
GTX34657 ICC/IF Image
ICC/IF analysis of MeOH-fixed HeLa cells using GTX34657 Cytokeratin 7 antibody [K72.7].
Green : Primary antibody
Red : nucleus
GTX34657 FACS Image
FACS analysis of PFA-fixed HeLa cells using GTX34657 Cytokeratin 7 antibody [K72.7].
Blue : Primary antibody
Red : Isotype control
GTX34657 IHC-P Image
IHC-P analysis of ovarian carcinoma tissue using GTX34657 Cytokeratin 7 antibody [K72.7].
GTX34657 IHC-P Image
IHC-P analysis of human lung scc tissue using GTX34657 Cytokeratin 7 antibody [K72.7].
GTX34657 WB Image
WB analysis of HeLa cell lysate using GTX34657 Cytokeratin 7 antibody [K72.7].