*Optimal dilutions/concentrations should be determined by the researcher.
|1-2μg/ml for 30 min at RT
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.
We do not recommend use of this product for Mouse,Rat,Chicken,Xenopus laevis.
10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Cytoskeletal preparation from HeLa cells
Protein A/G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
CARD2 , CK8 , CYK8 , K2C8 , K8 , KO , KRT8 , keratin 8 , Cytokeratin 8 , Keratin 8
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
GTX34669 WB Image
WB analysis of HCT116 cell lysate using GTX34669 Cytokeratin 8 antibody [C-43].
GTX34669 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX34669 Cytokeratin 8 antibody [C-43].
GTX34669 WB Image
WB analysis of MCF-7 cell lysate using GTX34669 Cytokeratin 8 antibody [C-43].