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DAB Chromogen/HRP Substrate kit

Cat No. GTX73338

Reference 1

Application

 IHC
Package
1 kit ($289)
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PRODUCT

Summary

DAB Chromogen/HRP Substrate kit - Liquid Format

APPLICATION

Application Note

Components:
1) 5mL concentrated amber-colored DAB Chromogen Solution.
2) 200mL clear Stable DAB/Plus Substrate Buffer.
3) One empty mixing dropper bottle.

Working Solution:
Aliquot 1mL of Stable DAB/Plus Buffer in mixing bottle. Add 20μL (one drop) of concentrated Stable DAB/Plus Chromogen. Replace tip and mix.

Testing Procedure:
1.) After peroxidase incubation, wash tissue sections with wash buffer.
2.) Wipe slides removing excess buffer. Add enough drops of working Stable DAB/Plus solution to cover tissue sections.
3.) Incubate for 5-10 minutes at room temperature.
For optimal results, observe reaction under the microscope for signal development. Once the desired signal to noise ratio is achieved, stop the reaction by washing slides in buffer.
Note: The working Stable DAB/Plus solution is stable for at least 2 weeks and should be prepared in an opaque bottle. Store at 2-8ºC when not in use. Any solution not used after this period should be discarded.

PROPERTIES

Form

Liquid

Storage

Keep as concentrated solution, aliquot and store at 4ºC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Peroxidase from the antibody detection system reacts with H2O2 substrate to degrade it,which then reacts with DAB, precipitating it at positive sites yielding a dark brown color.

REFERENCE

Application Reference

Xia X et al. Hindawi 2018; (Epub) Microtubule-associated protein 4 is a prognostic factor and promotes tumor progression in lung adenocarcinoma.
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Package List Price ($)
$ 289