DDDDK tag antibody [GT231]

Name: DDDDK tag antibody [GT231]
Brand: GeneTex
SKU: GTX629631
Price: 329.00 USD
Rating: 5 (1 reviews)

Anti-DDDDK tag antibody [GT231] used in Immunoprecipitation (IP). GTX629631

Anti-DDDDK tag antibody [GT231] used in Western Blot (WB). GTX629631

Cat No. GTX629631

Reference 4

Host	Mouse
Clonality	Monoclonal
Clone Name	GT231
Isotype	IgG2a
Application	WB, IP
Package	100 μl ($329), 25 μl ($169)

See all DDDDK tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000-1:10000
IP	1:100-1:500

Not tested in other applications.

Positive Control

DDDDK-tagged protein expressing 293T cells

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to DDDDK tag

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888126

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DDDDK tag antibody

Research Area

DATA IMAGES

GTX629631 IP Image

DDDDK tag antibody [GT231] immunoprecipitates DDDK-tagged protein in IP experiments.
IP Sample: 750 μg whole cell lysate/extract of DDDK-tagged protein expressing 293T cells.
A : 5 μg whole cell lysate/extract of DDDDK-tagged protein expressing 293T cells
B : Control with 2.5 μg of pre-immune mouse IgG
C : Immunoprecipitation of DDDK-tagged protein by 2.5 μg of DDDDK tag antibody [GT231] (GTX629631)
7.5% SDS-PAGE
The immunoprecipitated DDDK-tagged protein was detected by DDDDK tag antibody [GT231] (GTX629631) diluted at 1 : 5000.
EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

GTX629631 WB Image

DDDDK tag antibody [GT231] (GTX629631) detects DDDDK-tagged proteins by western blot.
A. Untransfected 293T whole cell lysate/extract (30 μg )
B.-D. Whole cell lysate/extracts (30 μg) from transfected 293T cells expressing different DDDDK-tagged proteins.
10% SDS-PAGE
DDDDK tag antibody [GT231] (GTX629631) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Zhang N et al. FASEB J 2020; 34 (11) : 15338-15363 N-glycosylation of CREBH improves lipid metabolism and attenuates lipotoxicity in NAFLD by modulating PPARα and SCD-1.

Application : WB

Chen KW et al. Biochim Biophys Acta 2016; 1860 (2) : 270-281 SH2B1 modulates chromatin state and MyoD occupancy to enhance expressions of myogenic genes.

Yuan W et al. Cancer Lett 2016; (Epub) CMTM3 Decreases EGFR Expression and EGF-Mediated Tumorigenicity by Promoting Rab5 Activity in Gastric Cancer.

Application : WB

Monjaret F et al. Mol Ther 2014; 22 (6) : 1176-87 Cis-splicing and translation of the pre-trans-splicing molecule combine with efficiency in spliceosome-mediated RNA trans-splicing.

Application : WB

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