DEPDC5 (phospho Ser1530) antibody

Cat. No. GTX04328

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ELISA
Reactivity	Human
Package	100 μl ($399)

See all DEPDC5 products

Datasheet

View product citations for antibody GTX04328 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:3000
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

181 kDa. ( Note )

Predict Reactivity

Zebrafish, Rabbit, Bovine, Dog, Monkey, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human DEPDC5 (Accession O75140), corresponding to amino acid residues around phosphorylated Ser1530.

Purification

Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DEP domain containing 5 , DEP.5 , FFEVF , FFEVF1

Cellular Localization

Cytoplasm, cytosol , Cytoplasm, perinuclear region , Lysosome membrane

Background

This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Database

Gene ID: 9681 DEPDC5
UniProt: O75140 DEPDC5

Research Area

DATA IMAGES

GTX04328 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using serial diluted GTX04328 DEPDC5 (phospho Ser1530) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

GTX04328 WB Image

WB analysis of HeLa cell lysate using GTX04328 DEPDC5 (phospho Ser1530) antibody. The lane on the right was treated with blocking peptide.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

Qty