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DLAT antibody [4A4-B6-C10]

Anti-DLAT antibody [4A4-B6-C10] used in Immunoprecipitation (IP). GTX49202
Anti-DLAT antibody [4A4-B6-C10] used in Western Blot (WB). GTX49202
Anti-DLAT antibody [4A4-B6-C10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49202

Cat No. GTX49202

Host

Mouse

Clonality

Monoclonal

Clone Name

4A4-B6-C10

Isotype

IgG1

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human DLAT protein fragments expressed in E.coli.

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Dihydrolipoamide S-Acetyltransferase , Dlta , Pdc-E2 , Pdce2 , Dlat

Cellular Localization

Mitochondrion matrix.

Background

This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood

Database

Research Area

DATA IMAGES

Anti-DLAT antibody [4A4-B6-C10] used in Immunoprecipitation (IP). GTX49202

GTX49202 IP Image

IP analysis of HeLa cell lysates using control IgG and DLAT antibody. The precipitates were detected by the same DLAT antibody.

Anti-DLAT antibody [4A4-B6-C10] used in Western Blot (WB). GTX49202

GTX49202 WB Image

WB analysis of indicated lysates using DLAT antibody at a dilution of 1:1,000.

Anti-DLAT antibody [4A4-B6-C10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49202

GTX49202 ICC/IF Image

ICC/IF analysis of HeLa cells using DLAT antibody at a dilution of 1:300.

REFERENCE

There are currently no references for DLAT antibody [4A4-B6-C10] (GTX49202). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for DLAT antibody [4A4-B6-C10] (GTX49202). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf