DLAT antibody [4A4-B6-C10]

Cat. No. GTX49202

Host	Mouse
Clonality	Monoclonal
Clone Name	4A4-B6-C10
Isotype	IgG1
Application	WB, ICC/IF, IP
Reactivity	Human, Mouse
Package	100 μg ($399)

See all DLAT products

Datasheet
Document

View product citations for antibody GTX49202 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IP	Assay dependent

Not tested in other applications.

Calculated MW

69 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human DLAT protein fragments expressed in E.coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dihydrolipoamide S-acetyltransferase , DLTA , E2 , PDC-E2 , PDCE2

Cellular Localization

Mitochondrion matrix

Background

This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.[provided by RefSeq, Oct 2009]

Database

Gene ID: 1737 DLAT
UniProt: P10515 DLAT

Research Area

DATA IMAGES

GTX49202 IP Image

IP analysis of HeLa cell lysates using control IgG and DLAT antibody. The precipitates were detected by the same DLAT antibody.

GTX49202 ICC/IF Image

ICC/IF analysis of HeLa cells using DLAT antibody at a dilution of 1:300.

GTX49202 WB Image

WB analysis of indicated lysates using DLAT antibody at a dilution of 1:1,000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty