Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody (GTX108820) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody (GTX108820) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using DNA ligase IV(GTX108820) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody (GTX108820) diluted at 1:500.

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX108820 diluted at 1:1000

DNA ligase IV antibody detects DNA ligase IV protein at nucleus and nucleolus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNA ligase IV stained by DNA ligase IV antibody (GTX108820) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNA ligase IV(GTX108820) antibody at 1:500 dilution.

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody (GTX108820) diluted at 1:500.

The data was published in the journal EMBO Mol Med in 2015. PMID: 25872942