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DNMT1 antibody [N1], N-term

Anti-DNMT1 antibody [N1], N-term used in Western Blot (WB). GTX116011
Anti-DNMT1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116011
Anti-DNMT1 antibody [N1], N-term used in ChIP assay (ChIP assay). GTX116011
Anti-DNMT1 antibody [N1], N-term used in Western Blot (WB). GTX116011
Anti-DNMT1 antibody [N1], N-term used in Western Blot (WB). GTX116011
Anti-DNMT1 antibody [N1], N-term used in Immunoprecipitation (IP). GTX116011
Anti-DNMT1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116011
Anti-DNMT1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116011
Anti-DNMT1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116011

Cat No. GTX116011

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

183 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Jurkat , Raji , NCI-H929

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human DNMT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036782

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DNA methyltransferase 1 , ADCADN , AIM , CXXC9 , DNMT , HSN1E , MCMT , m.HsaI

Cellular Localization

Nucleus

Background

DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNMT1 antibody [N1], N-term used in Western Blot (WB). GTX116011

GTX116011 WB Image

Wild-type (WT) and DNMT1 knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNMT1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116011

GTX116011 IHC-P Image

DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm and nucleus in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DNMT1 antibody [N1], N-term used in ChIP assay (ChIP assay). GTX116011

GTX116011 ChIP assay Image

Cross-linked ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or anti-DNMT1 antibody. The precipitated DNA was detected by PCR with primer set targeting to ILIR2.
ChIP experiment and primer designs are based on Cancer Res. 2009 Sep 15;69(18):7412-21.

Anti-DNMT1 antibody [N1], N-term used in Western Blot (WB). GTX116011

GTX116011 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000.

Anti-DNMT1 antibody [N1], N-term used in Western Blot (WB). GTX116011

GTX116011 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNMT1 antibody [N1], N-term used in Immunoprecipitation (IP). GTX116011

GTX116011 IP Image

DNMT1 antibody [N1], N-term immunoprecipitates DNMT1 protein in IP experiments.
IP samples: HeLa nuclear extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of DNMT1 protein by 4 μg DNMT1 antibody [N1], N-term (GTX116011)
5 % SDS-PAGE
The immunoprecipitated DNMT1 protein was detected by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-DNMT1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116011

GTX116011 ICC/IF Image

DNMT1 antibody [N1], N-term detects DNMT1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNMT1 protein stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:2000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Anti-DNMT1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116011

GTX116011 IHC-P Image

DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DNMT1 stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DNMT1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116011

GTX116011 IHC-P Image

DNMT1 antibody [N1], N-term detects DNMT1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
DNMT1 stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

DNMT1 antibody [N1], N-term Cat No. GTX116011

Rating ( Average 5 based on 6 users reviews)
Application
Western Blot(WB) ( Average 5 based on 4 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Diagn Pathol in 2012.PMID: 22455563
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Diagn Pathol in 2012.PMID: 22455563
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016.PMID: 27344963
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 25928539
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2018.PMID: 29416619
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2018.PMID: 29416619
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169