DNMT3B antibody

Wild-type (WT) and DNMT3B knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNMT3B antibody (GTX129127) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNMT3B antibody (GTX129127) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.

DNMT3B antibody detects DNMT3B protein at nucleus on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
DNMT3B antibody (GTX129127) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3B antibody (GTX129127) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunoprecipitation of DNMT3B protein from K562 nuclear extracts using 5 μg of DNMT3B antibody (GTX129127).
Western blot analysis was performed using DNMT3B antibody (GTX129127) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

DNMT3B antibody detects DNMT3B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNMT3B protein stained by DNMT3B antibody (GTX129127) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3B antibody (GTX129127) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Oncotarget in 2018. PMID: 29416619

The data was published in the journal Oncotarget in 2018. PMID: 29416619