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DNMT3B antibody

Anti-DNMT3B antibody used in Western Blot (WB). GTX129127
Anti-DNMT3B antibody used in Immunoprecipitation (IP). GTX129127
Anti-DNMT3B antibody used in Western Blot (WB). GTX129127
Anti-DNMT3B antibody used in IHC (Paraffin sections) (IHC-P). GTX129127
Anti-DNMT3B antibody used in Western Blot (WB). GTX129127
Anti-DNMT3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129127

Cat No. GTX129127

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

HeLa , HeLa nuclear , GFP-tagged DNMT3B-transfested 293T

Predict Reactivity

Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human DNMT3B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DNA methyltransferase 3 beta,ICF,ICF1,M.HsaIIIB

Cellular Localization

Nucleus

Background

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-DNMT3B antibody used in Western Blot (WB). GTX129127

GTX129127 WB Image

Wild-type (WT) and DNMT3B knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNMT3B antibody (GTX129127) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNMT3B antibody used in Immunoprecipitation (IP). GTX129127

GTX129127 IP Image

Immunoprecipitation of DNMT3B protein from K562 nuclear extracts using 5 μg of DNMT3B antibody (GTX129127).
Western blot analysis was performed using DNMT3B antibody (GTX129127).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-DNMT3B antibody used in Western Blot (WB). GTX129127

GTX129127 WB Image

DNMT3B antibody detects DNMT3B protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3B antibody (GTX129127) diluted by 1:500.
Observed TARGET protein has mutiple bands at 100 kDa, and 105 kDa, possibly due to alternative splicing. Reference: Mol Cell Biol. 2009 Oct;29(19):5366-76. and Nucleic Acids Res. 2001 Sep 1;29(17):3506-12.

Anti-DNMT3B antibody used in IHC (Paraffin sections) (IHC-P). GTX129127

GTX129127 IHC-P Image

DNMT3B antibody detects DNMT3B protein at nucleus on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
DNMT3B antibody (GTX129127) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DNMT3B antibody used in Western Blot (WB). GTX129127

GTX129127 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3B antibody (GTX129127) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-DNMT3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129127

GTX129127 ICC/IF Image

DNMT3B antibody detects DNMT3B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNMT3B protein stained by DNMT3B antibody (GTX129127) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169