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DNMT3L antibody [S117-9]

Anti-DNMT3L antibody [S117-9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX42020
Anti-DNMT3L antibody [S117-9] used in IHC (Paraffin sections) (IHC-P). GTX42020
Anti-DNMT3L antibody [S117-9] used in Western Blot (WB). GTX42020

Cat No. GTX42020

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 S117-9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μg ($339)
See all DNMT3L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Product Note

No cross-reactivity against DNMT3a or DNMT3B.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Fusion protein amino acids 1-387 of human DNMT3L, accession number BAA95556

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DNA methyltransferase 3 like

Cellular Localization

Nucleus

Background

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

Database

Research Area

DATA IMAGES

Anti-DNMT3L antibody [S117-9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX42020

GTX42020 ICC/IF Image

ICC/IF analysis of HaCaT cells using GTX42020 DNMT3L antibody [S117-9]. Staining in the nucleus, and accumulated aroudn the nucleus. Clear nuclear demarcations.
Fixation: Cold 100% methanol for 10 minutes at -20ºC.
Dilution : 1:100

Anti-DNMT3L antibody [S117-9] used in IHC (Paraffin sections) (IHC-P). GTX42020

GTX42020 IHC-P Image

IHC-P analysis of mouse backskin tissue section using GTX42020 DNMT3L antibody [S117-9]. Lower layers of the epidermis have positive cell staining in the cytoplasm.
Dilution : 1:100

Anti-DNMT3L antibody [S117-9] used in Western Blot (WB). GTX42020

GTX42020 WB Image

Western blot analysis of DNMT3L in a human tissue mix using a 1:1000 dilution of GTX42020.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 339