APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
No cross-reactivity against DNMT3a or DNMT3B.
PROPERTIES
Form
Liquid
Buffer
PBS pH7.4, 50% Glycerol
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Fusion protein amino acids 1-387 of human DNMT3L, accession number BAA95556
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
DNA methyltransferase 3 like
Cellular Localization
Nucleus
Background
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
Database
Research Area
DATA IMAGES
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GTX42020 ICC/IF Image
ICC/IF analysis of HaCaT cells using GTX42020 DNMT3L antibody [S117-9]. Staining in the nucleus, and accumulated aroudn the nucleus. Clear nuclear demarcations. Fixation: Cold 100% methanol for 10 minutes at -20ºC. Dilution : 1:100
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GTX42020 IHC-P Image
IHC-P analysis of mouse backskin tissue section using GTX42020 DNMT3L antibody [S117-9]. Lower layers of the epidermis have positive cell staining in the cytoplasm. Dilution : 1:100
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GTX42020 WB Image
Western blot analysis of DNMT3L in a human tissue mix using a 1:1000 dilution of GTX42020.
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REFERENCE
There are currently no references for DNMT3L antibody [S117-9] (GTX42020). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for DNMT3L antibody [S117-9] (GTX42020). Be the first to share your experience with this product.