GeneTex
United States (US)

DNase II antibody

DNase-II-antibody_IHC-P_GTX28119-1_18121410_919.jpg
DNase-II-antibody_WB_GTX28119-1_18121410_432.jpg

Cat No. GTX28119

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher

Calculated MW

40 kDa. ( Note )

Positive Control

Human spleen tissue lysate

Specificity/Sensitivity

Specific for the 32 kD alpha chain; does not recognise the 12 kD beta chain
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4 containing 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide - amino acids 347 - 360 of human DNase II

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus (1). DNase II causes both chromatin condensation and DNA fragmentation (1). The genes encoding human (2-4), porcine (4), and murine (5) DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (a) and 12kDa (b) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation (3). DNase II is ubiquitously expressed in human tissues.

Database

DATA IMAGES
DNase-II-antibody_IHC-P_GTX28119-1_18121410_919.jpg

GTX28119 IHC-P Image

Immunohistochemical staining ofDNaseII in human spleen with DNase II antibody (GTX28119) at 5μg/ml.

DNase-II-antibody_WB_GTX28119-1_18121410_432.jpg

GTX28119 WB Image

Western blot analysis of DNase II in human spleen tissue lysate in the absence (lane 1) or presence (lane 2) of blocking peptide with DNase II antibody (GTX28119) at 1μg/ml.

Package List Price ($)
$ 329