Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DUOX1 antibody [N1N3] (GTX119160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled A431 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DUOX1 antibody [N1N3] (GTX119160) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using DUOX1(GTX119160) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

The data was published in the journal PLoS One in 2017. PMID: 28099519