GeneTex
United States (US)

Defensin beta 1 (Active) recombinant protein

Cat No. GTX65070

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

Protein's bioactivity determined by its ability to chemoattract CD34+ dendritic cells using a concentration range of 0.1-1 ug/ml.

Calculated MW

5.0 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Buffer

20 mM PBS pH-7.4 and 130 mM sodium chloride

Storage

Reconstitute in ddH2O. Store at -20ºC. Product is stable for at least 12 months.

Antigen Species

Human

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥98%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Defensin Beta 1,Bd1,Defb-1,Defb101,Hbd1,Defb1

Background

The Defensin family are highly similar in their protein sequence and are microbicidal & cytotoxic peptides made by neutrophils. beta Defensin-1 (BD-1) is an antimicrobial peptide having the resistance of epithelial surfaces to microbial colonization. BD-1 has close proximity to Defensin alpha -1 and has been implicated in the pathogenesis of cystic fibrosis. Skin of patients having atopic dermatitis patients and mycosis fungoides (non-lesional and lesional) show lower human BD-1 mRNA expression and higher human BD-2 and human BD-3 mRNA expression. beta Defensin is highly expressed by epithelial cells. BD-1 may play a role in the pathogenesis of severe sepsis. Variation in human BD-1 contributes to asthma diagnosis, with apparent gender-specific effects. Human BD-3 is a dimer, while Human BD-1 and Human BD-2 are monomeric. Recombinant human beta Defensin-1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 47 amino acids and having a molecular mass of 5 kDa.

Research Area

Package List Price ($)
$ 259