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Dengue virus NS4B protein antibody

Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX103349
Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX103349
Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103349
Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103349
Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX103349
Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103349

Cat No. GTX103349

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, FACS

Reactivity

Dengue virus
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
FACS Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

Dengue virus 2-infected C6/36 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , *Dengue virus type 2 infected huh7

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Dengue virus NS4B protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240700

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Dengue Virus Non-structural protein 4B , Dengue Virus NS4B , DENV Non-structural protein 4B , DENV NS4B

Research Area

DATA IMAGES

Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX103349

GTX103349 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS4B protein antibody (GTX103349) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX103349

GTX103349 WB Image

Sample(15 μg of cell lysate)
A: BHK21
B: DV infected BHK21
10% SDS PAGE
GTX103349 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103349

GTX103349 ICC/IF Image

GTX103349 IF Image Immunofluorescence analysis of Denque virus 2-infected BHK-21, using Non-structural protein 4B (Dengue virus 2 ) antibody (GTX103349) at 1:2000 dilution.

Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103349

GTX103349 ICC/IF Image

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX103349) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX103349

GTX103349 WB Image

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extrac
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
12% SDS-PAGE
NS4B (Dengue virus) antibody (GTX103349) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103349

GTX103349 ICC/IF Image

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX103349) diluted at 1:2000.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

Dengue virus NS4B protein antibody Cat No. GTX103349

Rating ( Average 5 based on 5 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Flow cytometry(FACS) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 31138795
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 31138795
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 31138795
Date : Anonymous submitted on 03-May-2021
Score :
Application Tested : WB
Sample Species : DENV
Sample : A549 cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:2000, 4°C, 12Hr
Secondary Antibody : GTX213110-01, 1:2000, 27°C, 2Hr
Lane Description : Non-infected (mock) and infected (infection) A549 whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS4B protein antibody (GTX103349) diluted at 1:2000.
Date : Anonymous submitted on 15-Dec-2014
Score :
Application Tested : FACS
Sample Species : DENV
Sample : Dengue virus type 2 infected huh7 cells
Fixation : 2% paraformaldehyde in PBS
Permeabilization /Antigen retrieval : 0.1% Saponin, 2% FBS in PBS
Primary Antibody : 1:500, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169