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Dengue virus NS4B protein antibody

Orthogonal
Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX103349
Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103349
Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103349
Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX103349
Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103349
Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX103349
Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX103349
Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103349
Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX103349

Cat. No. GTX103349

Reference 9
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Dengue virus 2
Package
100 μl ($399),
25 μl ($169)
See all Dengue virus NS4B protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
FACS Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus 2-infected C6/36

Product Note

This antibody was raised against Dengue virus 2 NS4B protein, which may cross react with NS4B protein of Dengue virus 3.

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Dengue virus NS4B protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240700

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Dengue Virus Non-structural protein 4B , Dengue Virus NS4B , DENV Non-structural protein 4B , DENV NS4B

Research Area

DATA IMAGES

Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX103349

GTX103349 WB Image

Sample(15 μg of cell lysate)
A: BHK21
B: DV infected BHK21
10% SDS PAGE
GTX103349 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103349

GTX103349 ICC/IF Image

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX103349) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103349

GTX103349 ICC/IF Image

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX103349) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX103349

GTX103349 WB Image

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extrac
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
12% SDS-PAGE
NS4B (Dengue virus) antibody (GTX103349) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103349

GTX103349 ICC/IF Image

GTX103349 IF Image Immunofluorescence analysis of Denque virus 2-infected BHK-21, using Non-structural protein 4B (Dengue virus 2 ) antibody (GTX103349) at 1:2000 dilution.

Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX103349

GTX103349 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS4B protein antibody (GTX103349) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX103349

GTX103349 WB Image

Orthogonal

The data was published in the journal Cell Rep in 2019.PMID: 31067454

Anti-Dengue virus NS4B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103349

GTX103349 ICC/IF Image

The data was published in the journal Viruses in 2019.PMID: 31461934

Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX103349

GTX103349 WB Image

The data was published in the journal Cell Rep in 2019.PMID: 31067454

REFERENCE

Application Reference

Udawatte DJ et al. Front Cell Infect Microbiol 2022; (12) : 926036 Dengue virus downregulates TNFR1- and TLR3-stimulated NF-κB activation by targeting RIPK1
Application : WB
Shen TJ et al. JCI Insight 2022; 7 (21) Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein-mediated viral translation.
Application : WB
Tian JN et al. Viruses 2019; 11 (9) A Dengue Virus Type 2 (DENV-2) NS4B-Interacting Host Factor
Lin DL et al. Cell Rep 2019; 27 (6) : 1666-1674.e4 The ER Membrane Protein Complex Promotes Biogenesis of Dengue and Zika Virus Non-structural Multi-pass Transmembrane Proteins to Support Infection.
Application : WB, ICC/IF
Zhang J et al. Cell Host Microbe 2018; 23 (6) : 819-831.e5 Flaviviruses Exploit the Lipid Droplet Protein AUP1 to Trigger Lipophagy and Drive Virus Production.
Application : WB, ICC/IF
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REVIEW

Dengue virus NS4B protein antibody Cat. No. GTX103349

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Flow cytometry(FACS)
( Average 5 based on 1 users reviews)
Anti-Dengue virus NS4B protein antibody used in Western Blot (WB). GTX103349
Date : Anonymous submitted on 03-May-2021
Score :
Application Tested : WB
Sample : A549 cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:2000, 4°C, 12Hr
Secondary Antibody : GTX213110-01, 1:2000, 27°C, 2Hr
Lane Description : Non-infected (mock) and infected (infection) A549 whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS4B protein antibody (GTX103349) diluted at 1:2000.
Anti-Dengue virus NS4B protein antibody used in Flow cytometry (FACS). GTX103349
Date : Anonymous submitted on 15-Dec-2014
Score :
Application Tested : FACS
Sample Species : DENV2
Sample : Dengue virus type 2 infected huh7 cells
Fixation : 2% paraformaldehyde in PBS
Permeabilization /Antigen retrieval : 0.1% Saponin, 2% FBS in PBS
Primary Antibody : 1:500, 4°C, 16Hr
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169