Non-infected (–) and infected (+) BHK-21 whole cell extracts were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus prM protein antibody (GTX128093) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by PrM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

prM (Dengue virus) antibody detects PrM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

prM (Dengue virus 2) antibody detects prM (Dengue virus 2) protein by western blot analysis.
A.30 μg BHK-21 whole cell lysate/extract
B.30 μg whole cell lysate/extract of Dengue virus 2 infected BHK-21 cells
C.30 μg whole cell lysate/extract of JEV virus infected BHK-21 cells
15% SDS-PAGE
prM (Dengue virus 2) antibody (GTX128093) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

prM (Dengue virus) antibody detects prM (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: prM (Dengue virus) protein stained by prM (Dengue virus) antibody (GTX128093) diluted at 1:2000.
Blue: Hoechst 33342 staining.

The data was published in the journal PLoS Pathog in 2013. PMID: 24348242