E2F3 antibody [N2C3]

Name: E2F3 antibody [N2C3]
Brand: GeneTex
SKU: GTX102302
Price: 329.00 USD
Rating: 4 (1 reviews)

Anti-E2F3 antibody [N2C3] used in Western Blot (WB). GTX102302

Cat No. GTX102302

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human
Package	100 μl ($329), 25 μl ($169)

See all E2F3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000

Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T

Product Note

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Mouse, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human E2F3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240735

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

E2F transcription factor 3 , E2F-3

Background

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner. [provided by RefSeq]

Database

Gene ID: 1871 E2F3
UniProt: O00716 E2F3

Research Area

DATA IMAGES

GTX102302 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
10% SDS PAGE
GTX102302 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Hoareau-Aveilla C et al. Haematologica 2019; 104 (2) : 347-359 miR-497 suppresses cycle progression through an axis involving CDK6 in ALK-positive cells.

Chen PH et al. J Neurochem 2014; MicroRNA-302b-inhibited E2F3 transcription factor is related to all trans retinoic acid-induced glioma cell apoptosis.

Application : WB

Reactivity : Human

Submit a Reference

REVIEW